文献类型：期刊文献

英文题名：Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (Oreochromis niloticus)

作者：Huang, Yu[1,2,3];Wang, Zhiwen[1,2,3];Zheng, Qi[1,2,3];Tang, Jufen[1,2,3];Cai, Jia[1,2,3];Lu, Yishan[1,2,3];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China

年份：2018

卷号：72

起止页码：95

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000423644600012)、、WOS

基金：We are grateful to all the laboratory members for their constructive suggestions to improve the manuscript. This work was supported by National Natural Science Foundation of China and Science (Grant no. 31302226, 31572651), Technology Planning Project of Guangdong Province of China (grant no. 2015A020209181) and Excellent Doctoral Dissertation Breeding Project of Guangdong Ocean University (Grant no. 201602).

语种：英文

外文关键词：Oreochromis niloticus; CD28; CD80/86; Streptococcus agalactiae; Yeast two-hybrid

外文摘要：Interaction of CD28 with CD80 or CD86 molecules provides a costimulatory signals required in T cell activation. In this study, we cloned and analyzed a CD28 gene (On-CD28) and a CD80/86 gene (On-CD80/86) from Nile tilapia (Oreochromis niloticus). Sequence analysis revealed the typical characteristics of On-CD28 protein; for instance, the proline-based motif ((TYPPPL122)-T-117) is essential in binding of CD28 to CD80/86 ligands. Moreover, an extracellular Ig domain was found in On-CD80/86; this domain is responsible in binding of CD28 to CD80/86 receptors. Subcellular localization analysis showed that both On-CD28 and On-CD80/86 were distributed predominantly in the cytomembrane. Yeast two-hybrid assay showed that On-CD28 directly interacted with On-CD80/86. On-CD28 and On-CD80/86 transcripts were detected in all the examined tissues of healthy Nile tilapia, and the highest expression levels of On-CD28 and On-CD80/86 were detected in the brain and heart, respectively. Following a bacterial challenge using Streptococcus agalactiae in vivo, On-CD28 and On-CD80/86 were upregulated in head kidney, spleen, intestines, and brain. However, they showed different expression profiles in response to stimulation with inactivated S. agalactiae in vitro. These findings indicated that the interaction of On-CD28 with On-CD80/86 provides a costimulatory signals that possibly play an important role in T cell activation during S. agalactiae infection.