文献类型：期刊文献

中文题名：HPLC-ELSD法测定蛹虫草中6种游离氨基酸的含量

英文题名：Determination of Six Amino Acids in Cordyceps militaris by HPLC-ELSD

作者：王雅玲[1];代玲玲[2];马堃[2];赵轶男[2];李婷婷[2];孙力军[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]大连民族学院生命科学学院,辽宁大连116600

年份：2010

卷号：29

期号：10

起止页码：1091

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD、、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：国家自然科学基金资助项目(30972287)

语种：中文

中文关键词：高效液相色谱;微波提取;蛹虫草;蒸发光散射检测器

外文关键词：high performance liquid chromatography; microwave extraction; Cordyceps militaris; evaporative light-scattering detector

中文摘要：建立了反相高效液相色谱-蒸发光散射检测（HPLC-ELSD）直接测定6种未衍生游离氨基酸（脯氨酸Pro、缬氨酸Val、蛋氨酸Met、异亮氨酸Ile、亮氨酸Leu、苯丙氨酸Phe）含量的分析方法。采用VenusilMP-C18色谱柱,以甲醇和水为流动相进行线性梯度洗脱,流速为0.8mL·min-1,漂移管温度40℃,氮气流速2.5L·min-1;优化了流动相的洗脱条件。结果表明,在优化实验条件下,6种氨基酸峰面积的对数值与氨基酸质量浓度的对数值呈良好线性,检出限为10.0～15.0mg·mL-1,方法精密度及稳定性良好。采用该法对微波法提取的蛹虫草子实体中的氨基酸进行测定,待检蛹虫草样本中不含缬氨酸（Val）,其余5种氨基酸的总量为16.00mg/g,约占蛹虫草总质量的1.60%,其中脯氨酸（Pro）（占0.72%）和苯氨酸（Phe）（占0.56%）的含量较高。已检出的5种Pro、Met、Ile、Leu、Phe含量分别为7.20、0.88、0.69、1.65、5.58mg/g。

外文摘要：A high performance liquid chromatographic method coupled with evaporative light scattering detection（HPLC-ELSD）was developed for the determination of six underivatized free amino acids（Pro,Val,Met,Ile,Leu,Phe）.The sample was separated on a Venusil MP-C18 column using methanol-water as mobile phase by linear gradient elution.The optimal conditions were as the follows：flow rate：0.8 mL·min-1,drift tube temperature：40 ℃,nitrogen flow rate：2.5 L·min-1.The mobile phase elution conditions were optimized and the working system applicability was tested.The results showed that,under the optimal conditions,the logarithm correlation between peak area and amino acid concentration was in a good linearity.The detection limits of six amino acids were in the range of 10.0-15.0 mg·L-1.The method was applied in the detection of 6 amino acids in the fruiting body of Cordyceps militaris,and the results showed that except for Val,the other five amino acids（Pro,Met,Ile,Leu,Phe）were detected in the tested sample,and their total amount was 16.00 mg/g,accounting for 1.60% of the total mass of Cordyceps militaris,in which Pro（occupying 0.72% of the total mass of Cordyceps militaris）and Phe（occupying 0.56% of the total mass of Cordyceps militaris）were higher than the other three amino acids.The detected contents of five amino acids of Pro,Met,Ile,Leu and Phe were 7.20,0.88,0.69,1.65 and 5.58 mg/g,respectively.