文献类型：期刊文献

中文题名：提取琼胶糖的树脂新方法

英文题名：A new method for extracting agarose using basic resin

作者：张锐[1];孙美榕[2]

机构：[1]广东海洋大学现代生化中心,广东湛江524088;[2]深圳市天泰生物技术公司,广东深圳518101

年份：2006

卷号：25

期号：3

起止页码：28

中文期刊名：中国海洋药物

外文期刊名：Chinese Journal of Marine Drugs

收录：CSTPCD、、CSCD_E2011_2012、CSCD

语种：中文

中文关键词：琼胶糖;强碱性树脂;凝胶强度;结晶紫电泳;DNA凝胶电泳

外文关键词：agarose; basic resin; gel strength; gentian violet electrophoresis; DNA gel electrophoresis

中文摘要：目的研究一种低成本制备琼胶糖的简便方法。方法以江蓠琼胶粉为原料,采用NH4+型阳离子交换树脂脱除1.5%琼胶溶液中的金属离子,再用OH-型强碱性阴离子交换树脂在恒温70℃下吸附沉降琼胶酯,提取出白色琼胶糖。结果测定的灰分和硫酸根含量均符合电泳琼胶糖的指标,凝胶强度达到560g/cm2,提取率达57%,测定了其结晶紫电泳和DNA的琼胶糖凝胶电泳。结论本方法提取的琼胶糖具有良好的电泳性能,适合用于生物化学和分子生物学的凝胶电泳研究。本法与EDTA-2Na法和DEAE纤维素法相比,是一种低成本制备琼胶糖的简便方法。

外文摘要：Objective To study a new method for extracting agarose. Methods In 1.5% agar solution, adding the cation resin exchanged by NH4^＋ to take off metal ions, then using the OH basic resin to absorb agaropectin. Results and Conclusion The extracting rate was 57%, gel strength reached 560g/cm^2 , the agarose has good electricity function by measuring the gentian violet electrophoresis and the DNA gel electrophoresis, so it is suitable for biochemistry and molecular biology electrophoresis. It is a kind of simple and low cost method for extracting agarose which better than the EDTA-2Na method and DEAE cellulose method.