文献类型：期刊文献

英文题名：A novel bacteriocin PE-ZYB1 produced by Pediococcus pentosaceus zy-B isolated from intestine of Mimachiamys nobilis: Purification, identification and its anti-listerial action

作者：Zhang, Ying[1];Yang, Jingming[1];Liu, Ying[1];Wu, Yagian[1];Fang, Zhijia[1];Wang, Yaling[1];Sun, Lijun[1];Deng, Qi[1];Gooneratne, Ravi[2];Xiao, Lixia[3]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod, Coll Food Sci & Technol,Guangdong Higher Educ Ins, Zhanjiang 524088, Peoples R China;[2]Lincoln Univ, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand;[3]Yangzhou Univ, Sch Food Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China

年份：2020

卷号：118

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000503313900056)、、WOS

基金：This work was financially supported by Department of Science and Technology of Guangdong Province (NO. 2016A020222014, 2017A030303079, 2014A020217018), Guangdong Provincial Key Laboratory of Aquatic Product Processing and safety (NO. GDPKGAPPS1503). I also thank Prof. Ravi Gooneratne from Lincoln University, New Zealand for his technical assistance.

语种：英文

外文关键词：Novel bacteriocin; Purification; Action mode; Listeria monocytogenes; Pediococcus pentosaceus

外文摘要：A novel bacteriocin, PE-ZYB1, was produced by Pediococcus pentosaceus zy-B that was isolated from the intestine of Mirnachiamys nobilis and collected from the South China Sea. The bacteriocin PE-ZYB1 was purified using acetic ether extraction, Sephadex LH-20 column chromatography, sepharose cation exchange chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), in succession. Its molecular mass was determined to be MW 2019.22 Da, and its N-terminal amino acid sequence was DLFNKFNRSMQYGNGVE. The PE-ZYB1 was confirmed as a novel bacteriocin through a homology search, using UniProt BLAST (NO. A0A1Y0VLN8). Bacteriocin PE-ZYB1 was found to have extensive spectrum antimicrobial activity against both Grain-positive and Gram-negative bacteria. Bacteriocin PE-ZYB1 showed resistance to heat, was highly stable over pH 2-7 and was sensitive to trypsin. Increased extracellular electrical conductivity was detected for Listeria. monocytogenes treated by bacteriocin PE-ZYB1. Furthermore, bacteriocin PE-ZYB1 bactericidally destroyed the cell membrane integrity of L. monocyingenes, resulting in cell membrane perforation and permeabilization. These results indicate the potential of the bacteriocin PE-ZYB1 for use as a food bio-preservative.