文献类型：期刊文献

英文题名：Effects of dietary lysine on regulating GH-IGF system, intermediate metabolism and immune response in largemouth bass (Micropterus salmoides)

作者：Yang, Peng[1];Wang, Wenqiang[1];Chi, Shuyan[2];Mai, Kangsen[1];Song, Fei[1,3,4];Wang, Lei[1]

机构：[1]South China Normal Univ, Coll Life Sci, Inst Modern Aquaculture Sci & Engn IMASE,Guangdon, Guangzhou Key Lab Subtrop Biodivers & Biomonitori, Guangzhou 510631, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Guangdong, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524025, Peoples R China;[4]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China

年份：2020

卷号：17

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:000544613100011)、、WOS

基金：This research was supported by the Fund of Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW2019-06); the Research Start-Up Fund of South China Normal University. We thank Y.J. Xu and Z.Z. Sun for helping during the experiment and sampling.

语种：英文

外文关键词：Lysine; GH-IGF; Metabolism; Immune response; Largemouth bass (Micropterus salmoides)

外文摘要：Carnivorous fish have a high requirement for lysine to get optimal growth and health status. However, the mechanisms of lysine in regulating growth, intermediate metabolism and immune response have not been fully revealed in fish species. In this experiment, largemouth bass ( Micropterus salmoides ), a popular carnivorous fish, was chosen to explore the molecular response after the fish fed with lysine control diet (LC diet), low lysine diet (LL diet) and lysine supplementation diet (LS diet) for 8 weeks, respectively. Results showed that compared with the LC diet, the LL diet led to a reduction of plasma nutrients concentration and hypoactivated growth hormone-insulin like growth factor (GH-IGF) signaling pathway which further impaired lipogenesis and lipolysis. Lysine supplementation to the LL diet had effective effects on alleviating the repression of GH-IGF pathway and nutrient anabolism. Furthermore, the LL diet significantly activated kidney pro-inflammatory and anti-inflammatory responses. Lysine supplementation significantly suppressed these effects to the control level. The present study demonstrated that dietary lysine levels led to distinct GH-IGF response, which in turn regulated the lipid me-tabolism, pro-inflammatory and anti-inflammatory response. Our results provide a molecular basis for using lysine to improve nutrients metabolism and immune response in fish species.