文献类型：期刊文献

英文题名：Comparative Mucous miRomics in Cynoglossus semilaevis Related to Vibrio harveyi Caused Infection

作者：Zhao, Na[1,3];Jia, Lei[2];Li, Guangli[1];He, Xiaoxu[2];Zhu, Chunhua[1];Zhang, Bo[1,2]

机构：[1]Guangdong Ocean Univ, Guangdong Res Ctr Reprod Control & Breeding Techn, Southern Marine Sci & Engn Guangdong Lab Zhanjian, Fisheries Coll, Zhanjiang 524000, Peoples R China;[2]Tianjin Fisheries Res Inst, Tianjin 300200, Peoples R China;[3]Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Int Res Ctr Marine Biosci, Minist Educ,Ocean Univ, Shanghai 201306, Peoples R China

年份：2021

卷号：23

期号：5

起止页码：766

外文期刊名：MARINE BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000692659600001)、、EI(收录号：20213610858170)、Scopus(收录号：2-s2.0-85114136577)、WOS

基金：This work was supported by grants from the Tianjin Natural Science Foundation (17JCQNJC15000), transformation project of Tianjin Agricultural Achievements (201604090), special funding for Modern Agricultural Industrial Technology System (CARS-47-Z01), Modern Industrial Technology System in Tianjin (ITTFRS2017011).

语种：英文

外文关键词：Mucus; microRNAs; Infection; Vibrio harveyi; Cynoglossus semilaevis

外文摘要：Epidermal mucus is an important barrier and regulating mediator in fish. MicroRNAs (miRNAs) are proved to be involved in various biological processes, also as promising biomarkers for disease diagnosis. Vibrio harveyi has long been a noticeable bacterial pathogen in Cynoglossus semilaevis aquaculture. To find the evidence whether there are indicating miRNAs in mucus and whether the miRNAs are related to infections caused by V. harveyi, miRNA profiles of mucus from V. harveyi infected fish and healthy controls were screened by small RNA sequencing and verified by quantitative real-time PCR. This is the first report about miRNA profiling of flatfish mucus, aiming at illustrating the pathogenesis of V. harveyi caused infection and developing disease-related biomarkers. The results revealed significant differences in expression levels of some miRNAs between infected fish and healthy ones. Three hundred differentially expressed miRNAs were obtained after filtering through FC > 2 or FC < 0.5 and most of the differential miRNAs were downregulated. After verification through qRT-PCR, four unique miRNAs, dre-miR-451, dre-miR-184, dre-miR-205-5p > ssa-miR-205b-5p, and dre-miR-181a-5p > ssa-miR-181a-5p, were identified as V. harveyi infection-related signatures, consistent with sequencing trend. The expression levels of these four miRNAs in the infected fish were all significantly lower than controls. These miRNAs in mucus could be used to differentiate diseased and healthy fish in a non-invasive way with practical value for large-scale disease screening. They also provided new insights into the mechanism underlying the bacterial infections in fish.