文献类型：期刊文献

英文题名：Ovarian transcriptomic analysis and follicular development of Leizhou black duck

作者：Zou, Kun[1];Asiamah, Collins Amponsah[1];Lu, Li-li[1];Liu, Yuanbo[1];Pan, Yiting[1];Chen, Tongxin[1];Zhao, Zhihui[1];Su, Ying[1]

机构：[1]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524025, Peoples R China

年份：2020

卷号：99

期号：11

起止页码：6173

外文期刊名：POULTRY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000584401800103)、、Scopus(收录号：2-s2.0-85091762009)、WOS

基金：This work was sponsored by Guangdong Science and Technology Plan Project (2017A020208066), provincial-level major scientific research project in Guangdong Province (2017KZDXM043), the excellent dissertation cultivation project of Guangdong Ocean University (201610), the Postgraduate demonstration course construction project of Guangdong Ocean University (21411), National Natural Science Foundation of China (No. 30972085), and The Key Platform Project of Innovative Strong School Engineering by the Department of Education of Guangdong Province (2018302).

语种：英文

外文关键词：laying traits; Leizhou black duck; ovarian follicles; transcriptome sequencing

外文摘要：This study investigated the factors that caused the differences in egg production during the development of ovarian follicles in Leizhou black ducks. Leizhou black ducks population was divided into 2 groups as high-yield group (HG) and low-yield group (LG). The number of eggs (NE), age at first egg (AFE), weight at first egg, and egg weight (EW) of both groups were recorded, and differences were analyzed using the t test. The logistic model was used to simulate the egg production curves to analyze the production rules. The ovarian follicles of both duck groups were collected to count the number of different grades sized follicles, weigh the ovaries, and observe follicular sections to analyze the developmental differences. Ovarian transcriptomic sequencing was performed to investigate differentially expressed genes and signal pathways in both duck groups. The results revealed a significant difference (P < 0.01) in the NE laid, AFE, and EW between both groups. Comparatively, HG had significantly more (P, 0.01) large yellow follicles (LYF) than LG. The density of medullary layer cells of the follicle section was greater in HG than LG ducks. Transcriptome sequencing revealed a total of 1,027 differentially expressed genes between the HG and LG ducks of which 495 genes were upregulated, and 532 genes were downregulated. Fifty genes were related to reproduction and reproductive processes. Kyoto Encyclopedia of Genes and Genomes-enriched signaling pathways revealed 274 signal pathways enriched in these differentially expressed genes of which the steroid biosynthesis pathway was significantly enriched. Analysis (Q < 0.05) showed that HSD3 beta -> gonadotropin-releasing hormone (GnRH) and estrogen receptor (ESR) -> LH beta/ERK1/2 were enriched in the steroid biosynthesis signal pathway. Follicle-stimulating hormone signal pathway mediated by HSD3 beta -> GnRH and ESR -> LH beta/ERK1/2 may be involved in ovarian follicle development to regulate LYF reserve process and affect its ovulation cycle, which in turn influence the egg production of Leizhou black ducks.