文献类型：期刊文献

英文题名：Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt

作者：Hu, Han Qiao[1];Li, Xin Shen[2];He, Hong[1]

机构：[1]Guangdong Ocean Univ, Dept Biotechnol, Coll Agr, Zhanjiang 524088, Guangdong, Peoples R China;[2]S China Agr Univ, Coll Nat Resources & Environm, Dept Plant Pathol, Guangzhou 510640, Peoples R China

年份：2010

卷号：54

期号：3

起止页码：359

外文期刊名：BIOLOGICAL CONTROL

收录：SCI-EXPANDED(收录号：WOS:000280602100026)、、Scopus(收录号：2-s2.0-77955266907)、WOS

基金：This research was supported by the Open Project Program Foundation of the Engineering Research Center of Biological Control, Ministry of Education (20070207).

语种：英文

外文关键词：Endophytic bacteria; Heterologous expression; Mangrove; Ralstonia solanacearum

外文摘要：Understanding the mechanisms of the antagonistic endophytic bacteria is helpful in controlling plant diseases. An endophytic bacterium, Bg-C31, from mangrove was found to be antagonistic to some fungal and bacterial pathogens of plants and to be effective in the biocontrol of Capsicum bacterial wilt in pot and field trials. Bg-C31 was identified as Bacillus amyloliquefaciens by biochemical and physiological tests as well as sequences of 16S rDNA and the LCI gene. The antimicrobial substance produced by Bg-C31 was identified as a protein, which is resistant to protease k and heat, by ammonium sulfate precipitation and butanol extraction. The antagonistic gene was located in the chromosome by plasmid curing. A 29 kDa fusion protein of the LCI gene was expressed. Antimicrobial activity of the fusion protein to Ralstonia solanacearum was detected on gels in situ, indicating that the LCI gene could potentially be used to produce transgenic plants that are resistant to bacterial infection. (C) 2010 Published by Elsevier Inc.