文献类型：期刊文献

中文题名：细菌素CAMT2抗菌纳米纤维膜制备及其特性研究

英文题名：Preparation and characteristics of antibacterial nanofiber membrane containing bacteriocin CAMT2

作者：李啟彬[1];张雪梅[1];刘颖[1];房志家[1];徐春厚[2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东湛江524088;[2]广东海洋大学滨海农业学院,广东湛江524088

年份：2022

卷号：48

期号：21

起止页码：146

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：广东省科技计划项目(2016A020222014,2017A030303079)。

语种：中文

中文关键词：细菌素;静电纺丝;纳米纤维膜;抑菌;结构

外文关键词：bacteriocin;electrospinning;nanofiber;antibacterial;structure

中文摘要：该研究以解淀粉芽孢杆菌ZJHD3-06的细菌素CAMT2为抗菌活性成分,聚乙烯醇为包埋材料,采用静电纺丝技术制备CAMT2-聚乙烯醇(polyvinyl alcohol,PVA)抗菌纳米纤维膜,并对制得纳米纤维膜的抑菌活性、释放效果、结构以及低温下对常见水产基质中单增李斯特菌的抑制效果进行评价。结果表明,载药量为80 mg/mL的CAMT2-PVA纳米纤维膜抑菌活性保留效果良好,第0.5~2天为突释阶段,第2天进入缓释阶段,第7天后趋于稳定。扫描电镜显示纤维表面光滑平整,无串珠等不规则现象,纤维直径为720 nm;傅立叶红外光谱结果表明,电纺过程PVA对细菌素CAMT2实现了有效的物理包埋;CAMT2-PVA纳米纤维膜在3种典型的水产基质中抑菌效果显著。由此表明,CAMT2-PVA具有良好的潜在应用价值。

外文摘要：The bacteriocin CAMT2 from Bacillus amyloliquefaciens ZJHD3-06 was used as the antimicrobial active ingredient and polyvinyl alcohol(PVA) was as the encapsulation material.The antibacterial CAMT2—PVA nanofiber membranes were prepared by electrostatic spinning technique,and the inhibition activity,release effect,structure and inhibition effect of the produced nanofiber membranes against Listeria monocytogenes in common aquatic matrices at low temperature were evaluated.The results showed that the CAMT2—PVA nanofibers membranes with a drug loading of 80 mg/mL retained good antibacterial activity,with a quick release phase of 0.5-2 d,a slow-release phase on the second day and a stabilization phase after the seventh day.Scanning electron microscope showed that the surface of the fibers was smooth and flat,without irregularities such as beads,and the diameter of the fibers was 720 nm;the results of Fourier transform infrared showed that the bacteriocin CAMT2 was effectively encapsulated by PVA during the electrospinning process;the inhibition effect of CAMT2—PVA nanofibers membrane was significant in three typical aquatic substrates.This suggests that CAMT2—PVA has good potential applications.