文献类型：期刊文献

英文题名：Lactation Activity and Mechanism of Milk-Protein Synthesis by Peptides from Oyster Hydrolysates

作者：Chen, Suhua[1];Qin, Xiaoming[1,2];Zhang, Chaohua[1,2];Cao, Wenhong[1,2];Zheng, Huina[1,2];Lin, Haisheng[1,2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Ctr Shellfish Proc Zhanji, Key Lab Adv Proc Aquat Prod,Guangdong Prov Key La, Guangdong Higher Educ Inst,Natl Res & Dev Branch, Zhanjiang 524088, Peoples R China;[2]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2022

卷号：14

期号：9

外文期刊名：NUTRIENTS

收录：SCI-EXPANDED(收录号：WOS:000799312100001)、、Scopus(收录号：2-s2.0-85128747749)、WOS

基金：This study was supported by the China Agriculture Research System of MOF and MARA, China (CARS-49).

语种：英文

外文关键词：oyster (Crassostrea hongkongensis); mammary gland; prolactin; lactation; human mammary epithelial cells; milk-protein synthesis

外文摘要：Oyster meat has a tender texture and delicate flavor, and the oyster is an aquatic shellfish with high nutritional and economic values. As they are rich in protein, oysters serve as a good source for the preparation of bioactive peptides. However, research on the lactation effect and mechanism of the synthesis of polypeptides from oyster hydrolysates is yet to be observed. This study aimed to analyze the lactation activity of the fraction UEC4-1 and explore its mechanism. The results show that, in an in vivo experiment, UEC4-1 could significantly increase the concentration of PRL in the serum and mammary tissue and the concentration of PRLR in the mammary tissue in rats with postpartum hypogalactia. UEC4-1 promoted the development of mammary tissue structure, resulting in active lactation. UEC4-1 promoted the proliferation of MCF-10A in a dose-dependent manner and could significantly upregulate the gene expression levels of PRL, PRLR, CSN1S1, CSN2, CSN3 and CCND1. UEC4-1 could also significantly increase the expression of mTOR, AKT1, RPS6KB1 and STAT5A in MCF-10A and improve its phosphorylation level. These results show that UEC4-1 had the ability to upregulate the proliferation and PRL synthesis of MCF-10A and promote lactation. The ability of UEC4-1 to regulate the milk-protein synthesis signaling pathway is the mechanism behind this. Oysters had a remarkable effect on lactating mothers' sweating irritability after childbirth and may serve as an everyday diet to promote lactation. Postpartum dysgalactia is a common problem for lactating women. The study of the oyster's lactation-active peptide can provide dietary nutrition guidance for postpartum lactating mothers, and it has the potential to be used for the development of drugs for the treatment of postpartum hypogalactia or oligogalactia.