文献类型：期刊文献

英文题名：Autolysis of shrimp head by gradual temperature and nutritional quality of the resulting hydrolysate

作者：Cao, Wenhong[1];Zhang, Chaohua[1];Hong, Pengzhi[1];Ji, Hongwu[1];Hao, Jiming[1];Zhang, Jing[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524025, Guangdong, Peoples R China

年份：2009

卷号：42

期号：1

起止页码：244

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000260523100038)、、EI(收录号：20084111631671)、Scopus(收录号：2-s2.0-53149131675)、WOS

基金：This work was supported by the agriculture project of science & technology from Guangdong Department of Science Technology, China (No. 2007A020400001).

语种：英文

外文关键词：Shrimp head; Endogenous enzymes; Autolysis; Gradual temperature; Essential amino acid score

外文摘要：Autolysis method was investigated for protein recovery from shrimp head (SH) of white shrimp (Penaeus vannamei). Autolysis of SH by several temperature conditions (40 degrees C, 50 degrees C, 60 degrees C and gradual temperature) at its initial pH showed protein recovery of 43.6%, 73.6%, 50.3% and 87.4%, respectively. Autolysis of SH by gradual temperature (temperature was risen 5 degrees C every half an hour, from 40 degrees C to 70 degrees C served best for the preparation of autolysis hydrolysate (AH) with the maximum degree of hydrolysis (DH) 48.6% and the maximum protein recovery 87.4%. Subsequently, the chemical components of SH and its AH obtained by gradual temperature were investigated and comparably evaluated. Protein contents of SH and AH by gradual temperature method amounted to 60.6% and 88.8% (dry basis), respectively. Both SH and AH had high contents of tasting or functional amino acids. The contents of essential amino acids (EAA) of SH and AH were 340.26 and 405.30 mg/g protein, respectively. The amino acids scores of SH and AH were 73 and 96, respectively. The EAA of AH covered 96-100% of the FAO/WHO ideal protein. The high performance size exclusion chromatography of AH showed that with the increasing of DH values, there occurred a larger amount of smaller polypeptides with molecular weight <5000 Da. The nutritional value of SH could be greatly enhanced by gradual temperature autolysis. (C) 2008 Published by Elsevier Ltd on behalf of Swiss Society of Food Science and Technology.