文献类型：期刊文献

英文题名：Cysteine Inhibits the Growth of Fusarium oxysporum and Promotes T-2 Toxin Synthesis through the Gtr/Tap42 Pathway

作者：Qiu, Mei[1,2,3,4];Deng, Yijia[1,2,3,4];Deng, Qi[1,2,3,4];Sun, Lijun[1,2,3,4];Fang, Zhijia[1,2,3,4];Wang, Yaling[1,2,3,4];Huang, Xiaoyue[1,2,3,4];Zhao, Jian[5]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[2]Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[3]Guangdong Prov Engn Technol Res Ctr Marine Food, Zhanjiang, Peoples R China;[4]Guangdong Higher Educ Inst, Key Lab Adv Proc Aquat Prod, Zhanjiang, Peoples R China;[5]Univ New South Wales, Sch Chem Engn, Sydney, NSW, Australia

年份：2022

卷号：10

期号：6

外文期刊名：MICROBIOLOGY SPECTRUM

收录：SCI-EXPANDED(收录号：WOS:000879353100001)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (NSFC) (numbers 31871898 and 32172215), Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (numbers 2021KJ149 and 2021KJ151), Characteristic Innovation Project of Colleges and Universities in Guangdong Province (number 2020KQNCX025), Zhanjiang Competitive Allocation Project of Science and Technology Development Special Fund (numbers 200915134541577 and 2020A03009), and Guangdong Provincial Key Research and Development program(number 2021B0202060001).

语种：英文

外文关键词：amino acid; T-2 toxin; target of rapamycin complex 1 (TORC1) pathway

外文摘要：Fusarium oxysporum is ubiquitous and can easily contaminate food during processing and storage, potentially producing T-2 toxin, which can pose a severe health risk to public health. Previous research on the presence of T-2 has focused on starch-rich foods, while protein- and amino acid-rich foods have received relatively little attention. In this study, the effects of amino acids on the growth of F. oxysporum and its T-2 production were investigated by gene deletion and complementation experiments. The results showed that amino acids, including aspartic acid, methionine, isoleucine, serine, phenylalanine, and cysteine, significantly inhibited the growth of F. oxysporum, while promoting T-2 synthesis, with cysteine having the most pronounced effect. The target of rapamycin complex 1 (TORC1) is a key pathway in response to a variety of amino acids, including cysteine. gtr2 and tap42 were found to be negative regulators of T-2 synthesis. The study highlights the elevated risk of T-2 production by F. oxysporum in cysteine-rich foods and the need to take appropriate measures to prevent and control the potential harm that such foods may present to public health. IMPORTANCE F. oxysporum and its T-2 contamination of food not only leads to food wastage but also poses a major food safety challenge to humans. The growth and T-2 production characteristics of F. oxysporum in high-protein substrates are considerably different from those in grains. Here, we show that the abundant free amino acids in a protein-rich food matrix are a key regulatory factor for the growth of, and toxin production by, F. oxysporum. Cysteine has the most pronounced effect on inhibiting mycelial growth and promoting T-2 synthesis through the TORC1 pathway. This implies that consumers tend to overlook T-2 contamination due to the poor growth of F. oxysporum in food rich in protein and amino acids, especially cysteine. Therefore, particular attention should be paid to the protection of those products.