文献类型：期刊文献

英文题名：Significantly Accelerated Osteoblast Cell Growth on TiO₂/SrHA Composite Mediated by Phenolic Compounds (BHM) from Hippocampus kuda Bleeler

作者：Yuan, Qiong[1];He, Lei[2];Qian, Zhong-Ji[2,3];Zhou, Chunxia[1,3,4];Hong, Pengzhi[1,3,4];Wang, Zhe[5];Wang, Yi[6];Sun, Shengli[2];Li, Chengyong[2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[4]Guangdong Modern Agr Sci & Technol Innovat Ctr, Zhanjiang 524088, Peoples R China;[5]Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr Biomed Mat & Interfaces, Shenzhen 518055, Peoples R China;[6]Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon 999077, Hong Kong, Peoples R China

年份：2018

卷号：10

期号：36

起止页码：30214

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：SCI-EXPANDED(收录号：WOS:000444793000024)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (21874029), Science and Technology Planning Project of Guangdong Province (2016A020210114), Science and Technology Planning Project of Zhanjiang City (2016C01002), Industrial Development Special Fund of Dapeng New Area (KY20170209), Science and Technology Planning Project of Shenzhen City (JCYJ20170818111719650), Innovation and Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1), and Graduate Education Innovation Planning Project of Guangdong Ocean University (201838).

语种：英文

外文关键词：microstructure; strontium; hydroxyapatite; Hippocampus kuda Bleeler; osteoblast

外文摘要：The microstructure of hydroxyapatite is known to influence cellular behavior, can be used as a substrate for osteoblast growth, and exploited as a drug-release platform. However, easy delamination and self-decomposition of hydroxyapatite caused by poor adhesion with substrates are the main problems currently. In this paper, we successfully fabricated titanium dioxide/strontium-doped hydroxyapatite (TiO2/SrHA) composite scaffolds by self-generated strontium-substituted hydroxyapatite microspheres in TiO2 nano tubes. Moreover, the active compound 1-(5-bromo-2-hydroxy-methoxyphenyl)-ethanone (BHM) from Seahorse (Hippocampus kuda Bleeler) was loaded in this scaffold, and the controlled release kinetics of BHM was studied. It was found that in the first 5 h, the release concentration and time of BHM had a good linear relationship, and the correlation coefficient reached 0.98. TiO2/SrHA/BHM composites exhibited favorable cytocompatibility at a given concentration of BHM (20 mu mol/L). Compared to pure SrHA, TiO2 nanotubes, and traditional TiO2/SrHA composites, superior cytocompatibility (cell adhesion and proliferation) of MC3T3-E1 was obtained on TiO2/SrHA/BHM composites. The expression levels of osteogenic marker genes such as alkaline phosphatase, osteopontin, osteocalcin, runt-related transcription factor 2, and collagen I are also upregulated to varying degrees. This TiO2/SrHA composite scaffold-mediated phenolic compound BHM could be applied in bone tissue repair.