文献类型：期刊文献

英文题名：Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

作者：Yan, Tingting[1];Kong, Songzhi[1];Ouyang, Qianqian[1,2];Li, Chengpeng[1];Hou, Tingting[1];Chen, Yu[3];Li, Sidong[1]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650000, Yunnan, Peoples R China;[3]Beijing Inst Technol, Sch Mat Sci & Engn, Beijing 100081, Peoples R China

年份：2020

卷号：18

期号：5

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000541031900028)、、Scopus(收录号：2-s2.0-85084277175)、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 81803684), the Natural Science Foundation of Guangdong Province (No. 2018A030313416 and No. 2020A1515011011), the Project of Science and Technology Plan of Guangdong Province (No. 2015A020216019 and No. 2017A010103023), the Project of Enhancing School With Innovation of Guangdong Ocean University (No. GDOU2013050221 and No.GDOU2016050255), and the Team Project of Innovation and Entrepreneurship for Undergraduates of Guangdong Ocean University (No. CCTD201828).

语种：英文

外文关键词：chitosan-gentamicin conjugate; antimicrobial; anti-inflammatory; scald repair

外文摘要：Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day's culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 mu g/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 mu g/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing.