文献类型：期刊文献

英文题名：Multi-target interference of perillaldehyde at sub-inhibitory concentrations on quorum sensing in Serratia liquefaciens: attenuation of biofilm, virulence factors and AHL signaling molecules

作者：Zhang, Jinxia[1]; Liu, Shiqi[1]; Yan, Jiajun[1]; Liu, Shouchun[1]; Zhong, Saiyi[1]; Hong, Pengzhi[1]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Key Laboratory of Aquatic Products Processing and Safety, Guangdong Marine Food Engineering Technology Research Center, Guangdong Aquatic Prepared Food Processing and Quality Control Engineering Research Center, Guangdong Modern Agricultural Science and Technology Innovation Center, Guangdong, Zhanjiang, 524088, China

年份：2026

外文期刊名：SSRN

收录：EI(收录号：20260276987)

语种：英文

外文关键词：Biofilms - Mass spectrometry - Microwave integrated circuits - Molecular docking - Molecular modeling - Molecules - Physiology

外文摘要：Perillaldehyde (PAH), a natural monoterpene compound, was evaluated for its quorum-sensing (QS)-interfering activity against Serratia liquefaciens.In this study, MIC determination, biofilm assays, motility tests, virulence factor quantification, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and molecular docking were used to explore the inhibitory mechanism of PAH. The MIC of PAH against S. liquefaciens was 15 μL/mL. At sub-inhibitory concentrations, PAH significantly reduced biofilm formation, bacterial motility, extracellular protease activity, EPS production, and siderophore secretion in a dose-dependent manner. HPLC-MS/MS showed that PAH markedly decreased the level of the key QS signal molecule C?-HSL. Molecular docking revealed that PAH binds to the active pockets of SwrI and SwrR, occupies key residues, and interferes with the normal recognition and binding of C?-HSL, thus blocking the QS pathway and downregulating downstream virulence phenotypes. Overall, PAH effectively disrupts QS signaling and related physiological functions of S. liquefaciens, showing high potential as a green preservative for aquatic products. ? 2026, The Authors. All rights reserved.