文献类型：期刊文献

英文题名：Norethindrone alters growth, sex differentiation and gene expression in marine medaka (Oryzias melastigma)

作者：Dong, Zhongdian[1,2];Chen, Yuebi[1];Li, Xueyou[1];Zhang, Ning[1];Guo, Yusong[1];Liang, Yan-Qiu[3];Wang, Zhongduo[1,4]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ A, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[4]Hunan Normal Univ Sch, Coll Life Sci, State Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China

年份：2022

卷号：37

期号：5

起止页码：1211

外文期刊名：ENVIRONMENTAL TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000748505900001)、、EI(收录号：20220611584039)、Scopus(收录号：2-s2.0-85123899434)、WOS

基金：This research was funded by the National Natural Science Foundation of China (41806195, 41907346, 31972794), Special Innovation Projects of Colleges and Universities in Guangdong province (2018KTSCX087, 2018KQNCX110), Nanhai Scholar Project of GDOU (QNXZ201807, 201903), and State Laboratory of Developmental Biology of Freshwater Fish (2020KF004).

语种：英文

外文关键词：endocrine disruption; hypothalamic-pituitary-gonadal axis; marine medaka; masculinization; Norethindrone

外文摘要：Norethindrone (NET) is a widely used synthetic progestin, which appears in water environments and threatens aquatic organisms. In this study, marine medaka (Oryzias melastigma) larvae were exposed to 7.6 and 80.1 ng/L NET for 190 days. The effects of NET on growth, sex differentiation, gonad histology and transcriptional expression profiles of hypothalamic-pituitary-gonadal (HPG) axis-related genes were determined. The results showed that exposure to 80.1 ng/L NET caused an all-male marine medaka population and significantly decreased the growth of males. Exposure to 7.6 ng/L NET increased the ratio of males/females in the marine medaka population, decreased the growth of males and delayed the ovary maturation in females. However, the sperm maturation was accelerated by 7.6 or 80.1 ng/L NET. In females, the transcription levels of cytochrome P450 aromatase (cyp19a1a) and progesterone receptor (pgr) in ovaries, glucocorticoid receptor (gr) and vitellogenin (vtg) in livers were suppressed after exposure to 7.6 ng/L NET, which may cause delayed ovary maturation. In males, NET significantly decreased the transcription levels of follicle stimulating hormone beta (fsh beta) and Luteinizing hormone beta (lh beta)in the brain, Estrogen receptor beta (er beta)?gr and pgr in the liver, and vitellogenin receptor (vtgr) in the testes, while NET of 80.1 ng/L led to a significant up-regulation of steroidogenic acute regulatory protein (star) in the testes of males. These results showed that NET could influence growth, sex differentiation and gonadal maturation and significantly alter the transcriptional expression levels of HPG axis-related genes.