文献类型：期刊文献

英文题名：SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells

作者：Kang, Kai[1];Ma, Yao-Dan[1];Liu, Si-Qi[1];Huang, Ri-Wei[1];Chen, Jin-Jun[1];An, Li-Long[2];Wu, Jiang[2]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Dept Vet Med, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Coastal Agr Sci, Dept Anim Sci, Zhanjiang 524088, Peoples R China

年份：2023

卷号：15

期号：6

外文期刊名：VIRUSES-BASEL

收录：SCI-EXPANDED(收录号：WOS:001015651500001)、、Scopus(收录号：2-s2.0-85163944178)、WOS

语种：英文

外文关键词：SARS-CoV-2; blood-testis barrier; autophagy; Sertoli cells; junction protein; immune factor

外文摘要：The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) disrupts the blood-testis barrier (BTB), resulting in alterations in spermatogenesis. However, whether BTB-related proteins (such as ZO-1, claudin11, N-cadherin, and CX43) are targeted by SARS-CoV-2 remains to be clarified. BTB is a physical barrier between the blood vessels and the seminiferous tubules of the animal testis, and it is one of the tightest blood-tissue barriers in the mammalian body. In this study, we investigated the effects of viral proteins, via ectopic expression of individual viral proteins, on BTB-related proteins, the secretion of immune factors, and the formation and degradation of autophagosomes in human primary Sertoli cells. Our study demonstrated that ectopic expression of viral E (envelope protein) and M (membrane protein) induced the expressions of ZO-1 and claudin11, promoted the formation of autophagosomes, and inhibited autophagy flux. S (spike protein) reduced the expression of ZO-1, N-cadherin, and CX43, induced the expression of claudin11, and inhibited the formation and degradation of autophagosomes. N (nucleocapsid protein) reduced the expression of ZO-1, claudin11, and N-cadherin. All the structural proteins (SPs) E, M, N, and S increased the expression of the FasL gene, and the E protein promoted the expression and secretion of FasL and TGF-& beta; proteins and the expression of IL-1. Blockage of autophagy by specific inhibitors resulted in the suppression of BTB-related proteins by the SPs. Our results indicated that SARS-CoV-2 SPs (E, M, and S) regulate BTB-related proteins through autophagy.