文献类型：期刊文献

中文题名：红树植物秋茄根系固氮微生物群落的稳定性和复杂性及其关键影响因子研究

英文题名：Study on the stability and complexity of diazotrophic communities and key driving factors in the root-associated zones of the mangrove plant Kandelia obovata

作者：齐峰[1];邓晓杰[2];关永鹏[2];周盛垚[3];何庆[2];RAJAPAKSHALAGE Thashikala Nethmini[2];李楠[2];姜宫凌侠[2];陈清香[2];雷欣悦[2];侯庆华[2];黄来珍[2];李小蕾[2];韦巧艳[1]

机构：[1]桂林电子科技大学生命与环境科学学院,广西桂林541200;[2]广东海洋大学海洋与气象学院,广东湛江524088;[3]桂林理工大学环境科学与工程学院,广西桂林541006

年份：2026

卷号：45

期号：3

起止页码：153

中文期刊名：热带海洋学报

外文期刊名：Journal of Tropical Oceanography

收录：北大核心2023、、北大核心

基金：广东海洋大学创新团队(海洋灾害预警)项目(2023KCXTD015);广西科技计划项目(2024GXNSFBA010342);国家自然科学基金项目(42267018)。

语种：中文

中文关键词：固氮微生物;根系;红树林生态系统;共现网络;复杂性;稳定性

外文关键词：diazotrophic communities;root-associated zones;mangrove ecosystem;co-occurrence network;complexity;stability

中文摘要：红树植物秋茄根系固氮微生物物种组成丰富,对红树林生态系统氮循环至关重要,然而,关于固氮微生物在红树林生态系统中的生态适应机制,仍不十分清楚。本研究在中国南方的雷州红树林自然保护区分别采集了根内、根际和非根际各75份样品,基于高通量测序技术和分子生态学方法,研究了秋茄根内、根际、非根际的固氮微生物组成、多样性、复杂性和稳定性及其主要影响因子。研究发现,α变形菌纲(Alphaproteobacteria)、δ变形菌纲(Deltaproteobacteria)和γ变形菌纲(Gammaproteobacteria)是红树林根系中的优势物种,其中α变形菌在根内丰度最高(54.9%),显著高于根际(37.5%)与非根际(29.1%)。α多样性显示非根际Shannon指数最高,根际最低(P<0.05);NMDS(non-metric multidimensional scaling)与ANOSIM(analysis of similarities)分析(r=0.659,P<0.001)证实三个区域群落结构差异显著。共现网络分析显示,根际固氮微生物网络的鲁棒性最高,非根际连接最密,根内最复杂但最脆弱。Spearman相关性分析表明,环境因子对复杂性和稳定性的影响具有区域特异性:盐度与NH_(4)^(+)-N共同驱动非根际与根际的复杂性,NO_(3)^(-)-N主要驱动非根际稳定性;根内复杂性受总硫(total sulfur,TS)与NO_(2)^(-)-N显著负影响,而根际稳定性仅与盐度呈显著正相关。本研究加强了对红树林生态系统中固氮微生物复杂性和群落稳定性的维持机制的理解,对恢复和维持红树植被生态系统具有重要意义。

外文摘要：The root-associated diazotrophs of the mangrove species Kandelia obovata exhibit remarkable species richness,which are integral to nitrogen cycling within mangrove ecosystems.However,their ecological adaptation mechanisms remain inadequately understood.In this study,we collected 75 samples each of root endosphere,rhizosphere,and bulk soil from a mangrove nature reserve in Leizhou,South China.Based on high-throughput sequencing and molecular ecological approaches,we investigated the composition,diversity,complexity,and stability of diazotrophs across these three compartments of K.obovata,as well as their main influencing factors.The results showed that Alphaproteobacteria,Deltaproteobacteria,and Gammaproteobacteria were the dominant species in the mangrove root-associated diazotrophs,with Alphaproteobacteria having the highest abundance in the root endosphere(54.9%),significantly higher than that in the rhizosphere(37.5%)and bulk soil(29.1%).α-diversity analysis revealed that the Shannon index was the highest in the bulk soil and the lowest in the rhizosphere(P