文献类型：期刊文献

英文题名：Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A

作者：Xuan, Junyong[1];Wang, Zefu[1];Xia, Qiuyu[1];Luo, Tingyu[1];Mao, Qingya[1];Sun, Qinxiu[1];Han, Zongyuan[1];Liu, Yang[1];Wei, Shuai[1];Liu, Shucheng[1,2,3]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Guangdong Prov Engn Technol Res Ctr Seafood,Guang, Coll Food Sci & Technol,Guangdong Prov Engn Lab M, Zhanjiang 524088, Peoples R China;[2]Guangdong Lab Southern Marine Sci & Engn Zhanjian, Zhanjiang 524088, Peoples R China;[3]Dalian Univ Technol, Collaborat Innovat Ctr Key Technol Marine Food De, Dalian 116034, Peoples R China

年份：2022

卷号：11

期号：22

外文期刊名：FOODS

收录：SCI-EXPANDED(收录号：WOS:000887238400001)、、WOS

基金：This research was funded by the National Natural Science Foundation of China (32172252), Key Field Project Supported by Educational Commission of Guangdong Province (Biological Medicine and Health) (2022 ZDZX2028), and scientific research start-up funds of Guangdong Ocean University (R20077).

语种：英文

外文关键词：tuna oil; hydrolyzed acylglycerol; selective lipase; lipid modification; acylglycerol composition

外文摘要：Lipase hydrolysis is an effective method to develop different functional types of lipids. In this study, tuna oil was partially hydrolyzed at 30% and 60% by Thermomyces lanuginosus lipase (TL 100 L) and Candida Antarctica lipase A (ADL), respectively, to obtain lipid-modified acylglycerols. The lipidomic profiling of the acylglycerols was investigated by UPLC-Q-TOF-MS and GC-MS to clarify the lipid modification effect of these two lipases on tuna oil. The results showed that 247 kinds of acylglycerols and 23 kinds of fatty acids were identified in the five samples. In the ADL group, the content of triacylglycerols (TAG) and diacylglycerols (DAG) increased by 4.93% and 114.38%, respectively, with an increase in the hydrolysis degree (HD), while there was a decreasing trend in the TL 100 L group. TL 100 L had a better enrichment effect on DHA, while ADL was more inclined to enrich EPA and hydrolyze saturated fatty acids. Cluster analysis showed that the lipids obtained by the hydrolysis of TL 100 L and ADL were significantly different in the cluster analysis of TAG, DAG, and monoacylglycerols (MAG). TL 100 L has strong TAG selectivity and a strong ability to hydrolyze acylglycerols, while ADL has the potential to synthesize functional lipids containing omega-3 PUFAs, especially DAG.