文献类型：期刊文献

英文题名：A Systematic Analysis on mRNA and MicroRNA Expression in Runting and Stunting Chickens

作者：Zhang, Li[1,2,3];Li, Ying[1,2,4];Xie, Xiujuan[1,2];Xu, Haiping[1,2];Xu, Zhenqiang[1,2];Ma, Jinge[1,2];Li, Bixiao[1,2];Lin, Shudai[1,2];Nie, Qinghua[1,2];Luo, Qingbin[1,2];Zhang, Xiquan[1,2]

机构：[1]South China Agr Univ, Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Bree, Guangzhou 510642, Guangdong, Peoples R China;[2]South China Agr Univ, Minist Agr, Key Lab Chicken Genet,Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China;[4]Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China

年份：2015

卷号：10

期号：5

外文期刊名：PLOS ONE

收录：SCI-EXPANDED(收录号：WOS:000355183900084)、、Scopus(收录号：2-s2.0-84960155117)、WOS

基金：This work was supported by the Chinese Postdoctoral Science Foundation (2012M521611, 2013T60808), the National Natural Science Foundation (31301958), the Guangdong Provincial Natural Science Foundation (S2013010013382) and the China Agriculture Research System (CARS-42-G05). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文摘要：Runting and stunting syndrome (RSS), which is characterized by lower body weight, widely occurs in broilers. Some RSS chickens simply exhibit slow growth without pathological changes. An increasing number of studies indicate that broiler strains differ in susceptibility to infectious diseases, most likely due to their genetic differences. The objective of this study was to detect the differentially expressed miRNAs and mRNAs in RSS and normal chickens. By integrating miRNA with mRNA expression profiling, potential molecular mechanisms involved in RSS could be further explored. Twenty-two known miRNAs and 1,159 genes were differentially expressed in RSS chickens compared with normal chickens (P < 0.05). qPCR validation results displayed similar patterns. The differentially expressed genes were primarily involved in energy metabolism pathways. The antisense transcripts were extensively expressed in chicken liver albeit with reduced abundance. Dual-luciferase reporter assay indicated that gga-miR-30b/c directly target CARS through binding to its 30UTR. The miR-30b/c: CARS regulation mainly occurred in liver. In thigh muscle and the hypothalamus, miR-30b/c are expressed at higher levels in RSS chickens compared with normal chickens from 2 to 6 w of age, and notably significant differences are observed at 4 w of age.