文献类型：期刊文献

英文题名：Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the Mutant Aurantiochytrium sp.

作者：Liu, Liangxu[1,2,3];Hu, Zhangli[1,2,3];Li, Shuangfei[1,2,3];Yang, Hao[1,2,3];Li, Siting[1,2,3];Lv, Chuhan[1,2,3];Zaynab, Madiha[1,2,3];Cheng, Christopher H. K.[4];Chen, Huapu[5];Yang, Xuewei[1,2,3]

机构：[1]Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Technol Res Ctr Marine Algal Bioengn, Guangdong Key Lab Plant Epigenet, Shenzhen 518060, Peoples R China;[2]Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biol Resources & Ecol Env, Shenzhen Key Lab Microbial Genet Engn, Shenzhen 518055, Peoples R China;[3]Shenzhen Univ, Longhua Innovat Inst Biotechnol, Shenzhen 518060, Peoples R China;[4]Chinese Univ Hong Kong, Sch Biomed Sci, Hong Kong 999077, Peoples R China;[5]Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China

年份：2020

卷号：8

期号：4

外文期刊名：MICROORGANISMS

收录：SCI-EXPANDED(收录号：WOS:000533510400059)、、Scopus(收录号：2-s2.0-85083734217)、WOS

基金：This study was supported by National Key Research and Development Project (Grant No. 2018YFA0902500), the Natural Science Foundation of Guangdong Province (Grant No. 2018A030313139), Joint R&D Project of Shenzhen-Hong Kong Innovation (Grant No. SGLH20180622152010394), Hong Kong Innovation and Technology Commission TCFS (GHP/087/18SZ), and sponsored by the Shenzhen Taifeng East Marine Biotechnology Co. Ltd, Natural Science Foundation of Shenzhen (Grant No. KQJSCX20180328093806045), Shenzhen science and technology application demonstration project (Grant No. KJYY20180201180253571), and Shenzhen peacock plan (Grant No. 827-000192).

语种：英文

外文关键词：Docosahexaenoic acid (DHA); polyunsaturated fatty acids (PUFAs); mutant strain; Aurantiochytrium sp; transcriptome

外文摘要：Docosahexaenoic acid (DHA), a n-3 long-chain polyunsaturated fatty acid, is critical for physiological activities of the human body. Marine eukaryote Aurantiochytrium sp. is considered a promising source for DHA production. Mutational studies have shown that ultraviolet (UV) irradiation (50 W, 30 s) could be utilized as a breeding strategy for obtaining high-yield DHA-producing Aurantiochytrium sp. After UV irradiation (50 W, 30 s), the mutant strain X2 which shows enhanced lipid (1.79-fold, 1417.37 mg/L) and DHA (1.90-fold, 624.93 mg/L) production, was selected from the wild Aurantiochytrium sp. Instead of eicosapentaenoic acid (EPA), 9.07% of docosapentaenoic acid (DPA) was observed in the mutant strain X2. The comparative transcriptomic analysis showed that in both wild type and mutant strain, the fatty acid synthesis (FAS) pathway was incomplete with key desaturases, but genes related to the polyketide synthase (PKS) pathway were observed. Results presented that mRNA expression levels of CoAT, AT, ER, DH, and MT down-regulated in wild type but up-regulated in mutant strain X2, corresponding to the increased intercellular DHA accumulation. These findings indicated that CoAT, AT, ER, DH, and MT can be exploited for high DHA yields in Aurantiochytrium.