文献类型：期刊文献

英文题名：Molecular and functional characterization of interferon regulatory factor 1 (IRF1) in amphibian Xenopus tropicalis

作者：Gan, Zhen[1,2,3,4,5]; Cheng, Jun[1,3,4]; Hou, Jing[1,2,3,4,5]; Xia, Liqun[1,3,4]; Lu, Yishan[1,3,4]; Nie, Pin[2]

机构：[1] Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China; [2] State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; [3] College of Fishery, Guangdong Ocean University, Zhanjiang, 524025, China; [4] Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen, 518120, China; [5] Shenzhen Dapeng New District Science and Technology Innovation Service Center, Shenzhen, 518120, China

年份：2021

卷号：167

起止页码：719

外文期刊名：International Journal of Biological Macromolecules

收录：EI(收录号：20241015685383)、Scopus(收录号：2-s2.0-85097459658)

语种：英文

外文关键词：Antibiotics - Glycoproteins - Mammals - Transcription

外文摘要：Interferon regulatory factor 1 (IRF1) is an important regulator in controlling the transcription of type I interferon genes, and its functions have been well-characterized in mammals, birds and fish. However, little information is available regarding the function of amphibian IRF1. In this study, an IRF1 gene homolog named as Xt-IRF1 was identified in the Western clawed frog (Xenopus tropicalis), an amphibian model specie widely used for comparative immunology research. Xt-IRF1 and IRF1 in other vertebrates possess similar genomic structure and flanking genes, and were grouped together to form a separate clade in phylogenetic tree. In addition, Xt-IRF1 gene was constitutively expressed in all tissues examined, with the highest expression level observed in spleen, and was inducible after poly(I:C) stimulation. Importantly, the expression of Xt-IRF1 was markedly induced by recombinant type I interferon, and Xt-IRF1 induced a strong activation of both IFNβ and ISRE promoters. The present study opens the door to investigate the roles of IRF1 in amphibians, and thus contributes to a better understanding of the functional evolution of IRFs in lower tetrapods. ? 2020