文献类型：会议论文

英文题名：Affinity Changes of ssDNA Library in Vibrio SELEX by Two Detecting Methods

作者：Zheng, Jiang[1,2];Li, Jiaxiang[2];Li, Yubao[2];Li, Ting[2]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[2]Fujian Province Univ, Coll Fisheries, Key Lab Sci & Technol Aquaculture & Food Safety, Xiamen 3610201, Peoples R China

会议论文集：2nd International Conference on Biomedical Engineering and Informatics (BMEI)

会议日期：OCT 17-19, 2009

会议地点：Tianjin Univ Technol, Tianjin, PEOPLES R CHINA

主办单位：Tianjin Univ Technol

语种：英文

外文关键词：vibrio alginolyticus; SELEX; affinity detecting method; ssDNA library; ELISA; OD260

外文摘要：Affinity detection is a key step of the SELEX screening process for aptamer. Tradtional detection depends on the isotope or fluorescence marker in the ssDNA library, but isotope have the danger of radiation and fluorescence is not stable enough in some situations. Therefore, searching for a new, safe and proper detection for the affinity of ssDNA library is needed for the SELEX technology. In the present paper, two colorimetry analytic methods-ELISA and OD260 methods were explored and compared to study the affinity changes in the SELEX for aptamer against Vibrio alginolyticus. The results showed that the affintiy changed almost similarly in the two detecting ways. Comparatively, ELISA was a proper affintiy detecting method. Although the OD260 method was seemed to be more simple and available in the SELEX, influence of some impurities on the absorbance at 260nm could make it instable and limited its use.