文献类型：期刊文献

英文题名：Effects of a potential host gut-derived probiotic, Bacillus amyloliquefaciens AV5, on the growth, biochemical and metabolic responses, and disease resistance of Nile tilapia (Oreochromis niloticus)

作者：Shija V.M.; Chen H.; Huang J.; Masanja F.; Zhong Y.; Li Y.; Amoah K.; Zhimin J.; Ndandala C.B.; Cai J.

机构：[1]College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China;[2]Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China;[3]Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, 524088, China;[4]Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, China;[5]Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, 524002, China;[6]Guangxi Key Laboratory for Marine Natural Products and Combinational Biosynthesis Chemistry, Nanning, 530200, China;[7]Guangxi Beibu Gulf Marine Research Centre, Academy of Sciences, Guangxi, Nanning, 530007, China

年份：2025

卷号：595

外文期刊名：Aquaculture

收录：Scopus(收录号：2-s2.0-85206114403)

语种：英文

外文关键词：Disease resistance; Growth and biochemical responses; Metabolomics; Nile tilapia; Probiotic effects

外文摘要：The influence of the dietary probiotic Bacillus amyloliquefaciens AV5 on the growth, biochemical and metabolic responses, and disease resistance of Nile tilapia (Oreochromis niloticus) was evaluated. The commercial diet (GC), a diet containing 106 and 108 CFU/g of B. amyloliquefaciens AV5, denoted as G1 and G2, respectively, was fed for 30 days. Fish were allocated to 9 plastic tanks, and each diet was randomly assigned to triplicate groups of 40 fish (22.4 ± 1.3 g). After 30 days of the feeding trial, the specific growth rate, weight gain rate, and final weight increased significantly in G2 compared to those in GC and G1. Feed conversion ratio was significantly higher in the fish-fed GC group than in the G2 group. However, the survival rate of the fish ranged from 99.13 % to 97.17 %, with no significant difference. Glucose levels did not differ significantly (P < 0.05). Total antioxidant capacity (T-AOC), (superoxide dismutase (SOD), and catalase (CAT), as well as digestive enzymes (trypsin, lipase, and protease), were significantly increased in the G2 group relative to the GC and G1 groups. Significantly lower ALT, AST, CHO, TG, and MDA activities were observed in the G2 group than in the GC group. To evaluate the metabolomic response, PCA, OPLS-DA, and volcano plots revealed a distinct difference between the G2 and GC diets, suggesting that the G2 diet impacted the hepatopancreas of fish. Furthermore, challenge experiments revealed that fish fed B. amyloliquefaciens AV5 exhibited substantially increased resistance (P < 0.05) to S. agalactiae at a concentration of 1x108CFU/g. Therefore, dietary supplementation with B. amyloliquefaciens AV5 enhanced the growth performance, metabolic activity, and disease resistance of O. niloticus. ? 2024 Elsevier B.V.