文献类型：期刊文献

英文题名：Therapeutic effect and immune mechanism of chitosan-gentamicin conjugate on Pacific white shrimp (Litopenaeus vannamei) infected with Vibrio parahaemolyticus

作者：Liang, Fengyan[1,2];Sun, Chengbo[2];Li, Sidong[1];Hou, Tingting[1];Li, Chengpeng[1]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Dept Fisheries, Zhanjiang 524088, Peoples R China

年份：2021

卷号：269

外文期刊名：CARBOHYDRATE POLYMERS

收录：SCI-EXPANDED(收录号：WOS:000678037400001)、、EI(收录号：20212710596216)、Scopus(收录号：2-s2.0-85109104406)、WOS

基金：This work was financially supported by Natural Science Foundation of Guangdong Province, P.R.C (No. 2020A1515011011).

语种：英文

外文关键词：Chitosan; Litopenaeus vannamei; Vibrio parahaemolyticus; Non-specific immunity; Immune mechanism

外文摘要：To explore the disease resistance mechanism of chitosan conjugates, chitosan-gentamicin conjugate (CS-GT) was synthesized and systematically characterized, the immune mechanism of CS-GT on Litopenaeus vannamei infected with Vibrio parahaemolyticus was further explored. The results showed that imine groups in CS-GT were effectively reduced. Dietary supplementation of CS-GT can significantly increase the survival rate, total hemocyte counts, the antioxidant and immune related enzyme activity levels of shrimps (P < 0.05), which are all dosedependent under the experimental conditions. In addition, CS-GT can protect the hepatopancreas from invading bacteria and alleviate inflammation. Particularly, CS-GT promotes the expressions of legumain (LGMN), lysosomal acid lipase (LIPA) and Niemann-Pick type C2 (NPC2) up-regulated. It is speculated that CS-GT may stimulate the lysosome to phagocytose pathogens more effectively. In conclusions, shrimps fed with CS-GT can produce immune response via lysosome and greatly improve the disease resistance to Vibrio parahaemolyticus.