文献类型：期刊文献

英文题名：Structural characterization and antioxidant activity mechanism of the ferulic acid-rich subfraction from sugar beet pectin

作者：Xiang, Chunhong[1];Teng, Hui[1];Sheng, Zhili[2];Zhao, Chao[3];Deng, Jingteng[1];Zhao, Chengang[1];He, Bo[1];Chen, Lei[1];Ai, Chao[1]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Seafood,Guangd, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China;[3]Fujian Agr & Forestry Univ, Coll Marine Sci, Fuzhou 350002, Peoples R China

年份：2025

卷号：347

外文期刊名：CARBOHYDRATE POLYMERS

收录：SCI-EXPANDED(收录号：WOS:001309886500001)、、EI(收录号：20243617005843)、WOS

基金：This work was supported by grants from the National Natural Science Foundation of China (No. 32202028) , Guangdong Provincial Natural Science Foundation (No. 2024B1515020110) , and Guangdong Ocean University Scientific Research Start-up Funding Project (No. 060302042111) .

语种：英文

外文关键词：Sugar beet pectin; Structure characterization; Antioxidant; Gut microbiota; Caenorhabditis elegans

外文摘要：The feruloylated sugar chain in sugar beet pectin (SBP) is a natural polyphenol-polysaccharide complex. Its low abundance often leads to be neglected, thereby hindering its bioactivity and mechnism research. In this study, SBP-3 A, a novel feruloylated polysaccharide fragment, was isolated from sugar beet pectin utilizing enzymatic digestion. The presence of ferulic acid on SBP-3 A was confirmed through high-performance liquid chromatography (HPLC), with a mass fraction of 22.5 mu g/mg. The average molecular weight was determined to be 33.31 kDa. Methylation analysis, and nuclear magnetic resonance (NMR) spectra revealed that SBP-3 A is a heteroglycan with the main chain structure of -> 2)-alpha-Rhap-(1 -> 4)-alpha-GalpA-(1 -> 2)-alpha-Rhap-(1 ->, and the branched chain structure of ferulic acid (FA) -> 3,4)-(3-Galp-(1 -> 2,4)-alpha-Rhap-(1 ->. Subsequently, the antioxidant activity of SBP-3 A was evaluated using the Caenorhabditis elegans (C. elegans). SBP-3 A improved antioxidant enzymes and non-enzymatic defense system, decreased reactive oxygen species levels, and up-regulated the mRNA expression of sod-3, skn-1, and daf-16, while down-regulated the expression of age-1 in C. elegans. Moreover, SBP-3 A modulated the gut flora by favorably affecting the abundances of Lactobacillus, Ligilactobacillus, and Akkermansia, thereby enhancing antioxidant capacity in C. elegans. Consequently, the aforementioned findings support the potential application of SBP-3 A as a functional food for treating oxidative stress-related illnesses.