文献类型：期刊文献

英文题名：The properties and formation mechanism of ovalbumin-fucoidan complex

作者：Duan, Wenshan[1,2];Chen, Lei[3];Liu, Fei[1];Li, Xiefei[1];Wu, Yongyan[1,2];Cheng, Lujie[1,2];Liu, Junmei[1,4];Ai, Chao[3];Huang, Qun[1,2,4];Zhou, Yan[1]

机构：[1]Guizhou Med Univ, Guizhou Prov Engn Res Ctr Hlth Food Innovat Mfg, Sch Publ Hlth, Key Lab Environm Pollut Monitoring & Dis Control,M, Guiyang 550025, Peoples R China;[2]Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Seafood, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[4]Chengdu Univ, Inst Egg Sci & Technol, Sch Food & Biol Engn, Chengdu 610106, Peoples R China

年份：2023

卷号：241

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:000998991100001)、、EI(收录号：20242616348711)、Scopus(收录号：2-s2.0-85154026934)、WOS

基金：This study was financially supported through grants from the Key Project of Natural Science Foundation of Guizhou Province (No. KY [2022] key 036) , the National Natural Science Foundation of China (No. 32260597) , the Open Foundation for Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education (No. KY [2022] 443) , and the Foundation of Guizhou Educational Committee (No. KY [2021] 008) .

语种：英文

外文关键词：Ovalbumin; Fucoidan; Electrostatic interactions; Soluble complexes

外文摘要：The polymeric materials formed by proteins and polysaccharides through molecular interactions have attracted public attention. In this study, a novel binary complex consisting of ovalbumin (OVA) and fucoidan (FUC) was obtained by electrostatic self-assembly. The self-assembly properties and the formation mechanism of the OVA-FUC binary complex were investigated by changing the charging degree and density of complex through altering pH value and polysaccharides proportion. Structural changes during the OVA-FUC electrostatic self-assembly process were investigated by a phase diagram, zeta-potential, and particle size. The optimal conditions for pre-paring soluble OVA-FUC binary complex were determined by the protein retention rate and insoluble solids content. Results showed that the soluble OVA-FUC binary complex could be obtained at the pH of 3.5 to 5, and the insoluble OVA-FUC binary complex was generated at the pH of 2.5 to 3.5. The OVA-FUC binary complex (19 +/- 0.29 mN/m) possessed a medium ability to reduce interfacial tension of the water-oil interface compared with OVA (15 +/- 1.13 mN/m) and FUC (24 +/- 0.3 mN/m), indicating that OVA-FUC binary complex has good amphiphilicity and can be applied as a potential pH-controlled emulsifier in function food systems for delivering bioactive substances.