文献类型：期刊文献

中文题名：低盐度下金钱鱼对嗜水气单胞菌感染的生理生化及免疫响应

英文题名：Physiological,Biochemical and Immune Responses to Aeromonas hydrophila Infection in Spotted Scat(Scatophagus argus)Under Low Salinity Conditions

作者：冷卓颖[1,2];蔡博生[1,2];吴天利[1,2];黄洋[1,2];庞欢瑛[1,3];李广丽[1,2];邓思平[1,2]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]广东海洋大学广东省名特优鱼类生殖调控与繁育工程技术研究中心,广东湛江524088;[3]广东海洋大学广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088

年份：2025

卷号：32

期号：2

起止页码：20

中文期刊名：海南热带海洋学院学报

外文期刊名：Journal of Hainan Tropical Ocean University

基金：国家自然科学基金项目(31972775);广东海洋大学科研启动经费项目(060302022311);广东省科技计划项目(2023B0202010005)。

语种：中文

中文关键词：金钱鱼;嗜水气单胞菌;血清指标;免疫响应

外文关键词：Scatophagus argus;Aeromonas hydrophila;serum parameters;immune response

中文摘要：为研究金钱鱼(Scatophagus argus)在低盐度养殖条件下对嗜水气单胞菌(Aeromonas hydrophila)感染的响应情况,本研究随机选择体质量为(200±50)g、体长为(13.5±0.5)cm的金钱鱼,在盐度为5‰的养殖条件下进行嗜水气单胞菌腹腔注射感染,并在感染后0、3、6 h检测血清生理生化和免疫指标;同时使用实时荧光定量PCR检测头肾和脾脏中与免疫相关基因的表达情况。结果显示:在注射嗜水气单胞菌3、6 h后,处理组金钱鱼血清中的补体C3、补体C4、免疫球蛋白IgM含量和溶菌酶LZM活性相比对照组显著增加(P<0.05),血糖GLU含量显著降低(P<0.05)。但处理组超氧化物歧化酶SOD活性仅在6 h显著增加(P<0.05),谷草转氨酶AST活性仅在3 h显著降低(P<0.05),碱性磷酸酶AKP活性仅在6 h显著降低(P<0.05)。此外,处理组血清中的谷丙转氨酶ALT、酸性磷酸酶ACP、过氧化物酶POD活性和甘油三酯TG、总胆固醇TC、总蛋白TP含量相比对照组无显著差异。注射嗜水气单胞菌3、6 h后,处理组金钱鱼头肾和脾脏组织中的il-1β、il-10、tnf-α、socs1基因的相对表达量比对照组显著增加(P<0.05)。

外文摘要：To investigate the response of spotted scats to A.hydrophila infection under low salinity culture conditions,spotted scats with a body weight of 200±50 g and body length of 13.5±0.5 cm were randomly selected for A.hydrophila intraperitoneal injection at a salinity of 5‰.Serum physiological,biochemical,and immunological indexes were measured at 0,3,and 6 hours postinfection.In addition,the expression of genes related to immunity in the head,kidney,and spleen was detected by real-time quantitative fluorescence PCR.The results showed that 3 and 6 hours after A.hydrophila injection,the serum levels of complement C3 and complement C4,the content of immunoglobulin M(IgM),and lysozyme(LZM)activities in the treatment group significantly increased(P<0.05),and the content of blood glucose(GLU)significantly decreased(P<0.05)compared with those of the control group.However,superoxide dismutase(SOD)activity in the treatment group significantly increased(P<0.05)only at 6 h,aspartate transaminase(AST)activity decreased(P<0.05)only at 3 h,and alkaline phosphatase(AKP)activity significantly decreased only at 6 h(P<0.05).There were no significant differences in serum activities of acid alanine aminotransferase(ALT),acidphosphatase(ACP)and peroxidase(POD),and the contents of triglyceride(TG),total cholesterol(TC)and total protein(TP)in the treatment group compared to the control group.After 3 and 6 hours of A.hydrophila injection,the relative expression of interleukin-1β(il-1β),interleukin-10(il-10),tumor necrosis factor-α(tnf-α),and suppressor of cytokine signaling 1(socs1)genes in head,kidney and spleen tissues of spotted scat in the treatment group significantly increased(P<0.05)compared with that of the control group.