文献类型：期刊文献

中文题名：抗菌豆豉发酵菌株的筛选及其脂肽组分鉴定和特性研究

英文题名：Identification of Antimicrobial Lipopetides Component Produced by Isolate from Douchi and Its Antimicrobial Properties

作者：孙力军[1];王雅玲[1];刘唤明[1];徐德峰[1];张永平[1];聂芳红[1]

机构：[1]广东省水产品加工与安全重点实验室广东海洋大学食品科技学院

年份：2013

期号：7

起止页码：50

中文期刊名：中国生物工程杂志

外文期刊名：China Biotechnology

收录：CSTPCD、、北大核心2011、北大核心、CSCD、CSCD2013_2014

基金：国家自然科学基金(30972287);"十二五"国家支撑计划项目(2012BAD29B06);广东省教育部产学研结合项目(2011B090400154);湛江科技计划(2011D02)资助项目

语种：中文

中文关键词：豆豉;纳豆菌;抗菌活性;抗菌脂肽

外文关键词：Douchi Bacillus subtilus Antimicrobial activity Antimicrobial lipopeptide

中文摘要：目的:从豆豉中分离广谱抗菌活性菌株,并鉴定其发酵产物中的抗菌成分。方法:采用生理生化实验结合16SrRNA序列测定法鉴定目标分离株,通过LC-MS和ESI/CID技术对其抗菌组分进行鉴定。结果:从8个豆豉样品中分离出7株具有抗菌活性菌株,其中抗菌活性较强的分离株NT-6为枯草芽孢杆菌(Bacillus subtilis),其发酵液抗菌提取物的主要成分是抗菌脂肽类物质iturin、fengycin、surfactin同系物的混合物。该提取物对供试革兰氏阳性、阴性细菌、霉菌等指示菌具有广谱的抗菌活性,在pH 2～12、121℃条件下加热30min活性不丧失,说明具有广泛的pH适应范围和良好的耐热性。结论:该菌株产生多组分抗菌脂肽,其抑菌谱宽,适应性好,显示了在食品、农业、医药等领域具有良好的应用前景。

外文摘要：Objective:The paper report that screening broad-spectrum antimicrobial activity strain from douchi and identification of antimicrobial component of fermented extracts produced by the isolate.Methods: Through physiological and biochemical experiments and 16SrRNA sequencing analysis,the isolate identification was carried out.Analysis of the related antimicrobial substances was carried out by high performance liquid chromatography(HPLC)-electrospray ionization(ESI)-mass spectrometer(MS).Results: Seven antimicrobial activity isolates were isolated from eight douchi samples.The isolate,strain NT-6 with stronger antimicrobial activity,was identified as Bacillus subtilus,which produced three families of antimicrobial lipopeptides,iturins and fengycins and surfactins.Culture filtrate extracts of strain NT-6 strongly inhibited the growth of all Gram-positive bacteria tested except Bacillus subtilus and all Gram-negative ones and all fungi except Fusarium oxysporum f.sp.vasinfectum.And,antimicrobial activity of the extracts was stable under pH 2~12 and 121℃ 30min conditions.Conclusion: Therefore,it shows good application prospects in the food,agriculture,medicine and other fields that the isolate can produce more antimicrobial lipopeptide ingredients,a wide antimicrobial spectrum,and good adaptability.