文献类型：期刊文献

中文题名：砷脂促进秀丽隐杆线虫衰老的毒性效应及其分子机制

英文题名：Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans

作者：尹欢[1];宋兵兵[1];黎梓玉[1];汪卓[1];赵巧丽[1];李瑞[1];陈建平[1];钟赛意[1,2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,广东省亚热带果蔬加工科技创新中心,广东湛江524088;[2]广东海洋大学深圳研究院,广东深圳518108

年份：2024

卷号：45

期号：23

起止页码：131

中文期刊名：食品科学

外文期刊名：Food Science

收录：北大核心2023、CSTPCD、、EI(收录号：20245017517079)、Scopus、CSCD2023_2024、北大核心、CSCD

基金：国家自然科学基金面上项目(31972163);广东省高校重点领域专项(2023ZDZX2025);广东省高校科技创新团队项目(2021KCXTD021)。

语种：中文

中文关键词：砷脂;有机砷;毒性;衰老;分子机制;秀丽隐杆线虫

外文关键词：arsenolipids;organic arsenic;toxicity;aging;molecular mechanism;Caenorhabditis elegans

中文摘要：以秀丽隐杆线虫(Caenorhabditis elegans)为模型,研究AsHC 332和AsHC 346等常见于海产品中两种不同分子质量砷脂对线虫衰老和寿命相关指标的影响,并通过测定对线虫DAF-16蛋白表达情况、衰老突变株寿命和应激相关基因的影响分析其促衰老的可能机制。结果表明,砷脂以浓度依赖性的方式显著降低了线虫体长、体宽、后代数目和头部摆动频率(P<0.05);降低了线虫对热应激(P<0.0001)和紫外应激抗逆性(P<0.05);显著缩短了线虫寿命(P<0.01);增加了线虫脂褐素积累(P<0.001);上调了daf-16、sod-3、ctl-1、gst-4和hsp-16.2基因相对表达,对线虫DAF-16蛋白核定位促进作用不显著,对daf-16(mu86)突变株寿命无影响。研究表明AsHC 332和AsHC 346对线虫衰老的毒性效应相同,可能依赖于DAF-16转录因子的调节,从而缩短线虫寿命。

外文摘要：In this study,we explored the effect of two arsenolipids with different molecular masses,AsHC 332 and AsHC 346,which are commonly found in seafood,on aging and lifespan-related indicators in the model organism Caenorhabditis elegans.The possible mechanism by which the arsenolipids promote aging was analyzed by measuring the expression of DAF-16 protein in the nematode,the longevity of aging mutants and stress-related genes.The results showed that AsHCs significantly reduced the body length,body width,brood size and head thrash frequency of C.elegans in a dose-dependent manner(P<0.05).Moreover,the resistance to heat stress(P<0.0001)and ultraviolet stress(P<0.05)was significantly reduced by AsHCs.The lifespan of C.elegans was significantly shortened(P<0.01).The accumulation of lipofuscin was increased(P<0.001).The relative expression of the daf-16,sod-3,ctl-1,gst-4 and hsp-16.2 genes was up-regulated,while the nuclear localization of DAF-16 was not significantly promoted,and there was no effect on the lifespan of the daf-16(mu86)mutant.AsHC 332 and AsHC 346 had the same toxic effects on nematode aging,which may shorten the lifespan of C.elegans by regulating the transcription factor DAF-16.