文献类型：期刊文献

英文题名：Growth performance, antioxidant ability, biochemical index in serum, liver histology and hepatic metabolomics analysis of juvenile hybrid grouper (♀ Epinephelus fuscoguttatus x ♂ Epinephelus lanceolatus) fed with oxidized fish oil

作者：Long, Shuisheng[1,2];Dong, Xiaohui[1,2,3];Tan, Beiping[1,2,3];Zhang, Shuang[1,2,3];Xie, Shiwei[1,2];Yang, Qihui[1,2,3];Chi, Shuyan[1,2,3];Liu, Hongyu[1,2,3];Deng, Junming[1,2];Yang, Yuanzhi[1];Zhang, Haitao[3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Nutr & Feed, Zhanjiang 524088, Guangdong, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang 524088, Guangdong, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang 524000, Guangdong, Peoples R China

年份：2021

卷号：545

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000691824800002)、、WOS

基金：This study was supported by the Nation Natural Science Fund (31972808) , Natural Science Foundation of Guangdong Province (2021A1515011165) , the China Agriculture Research System (CARS-47) , and the "Chong Yi Liu (231419011) " project of Guangdong Ocean University, Science and Technology Bureau of Zhanjiang (2020A03010 & 2020A05003) .

语种：英文

外文关键词：female Epinephelus fuscoguttatus x male Epinephelus; lanceolatus; Oxidative stress; Growth performance; Liver histology; Metabolomics

外文摘要：Fish oil oxidation is a common phenomenon in aquaculture and produces harmful substances that can cause oxidative stress to aquatic animals. However, the metabolic mechanism of oxidized fish oil in the liver of hybrid groupers (female Epinephelus fuscoguttatus x male Epinephelus lanceolatus) is still unclear. In this study, a rearing experiment was conducted for 65 days with diets containing 0, 3, 6, and 9% oxidized fish oil (referred to as R, L, M, and H respectively) to investigate the effect of oxidative stress on growth performance, liver antioxidant status, biochemical index in serum, liver fat accumulation and hepatic metabolism profile of juvenile hybrid groupers. The results showed the oxidized fish oil inhibited the growth performance of grouper, and the growth performance became worse as the proportion of oxidized fish oil in the diet increased. Meanwhile, oxidized fish oil caused oxidative stress in the liver by reducing antioxidant enzyme activity and increasing the content of malondialdehyde. In addition, oxidative stress disrupted lipid metabolism by lowering low-density lipoproteins and increasing elevated-density lipoproteins in serum, and promoted the formation of fatty livers. To explore the mechanism of oxidative stress in the liver, the samples in the fresh fish oil group and the highest oxidized fish oil group were selected for non-targeted metabolome analysis. The differential metabolites in the livers were mainly phosphatidylcholines, phosphatidylethanolamines, and lysophosphatidylchoines with an upregulated status under oxidative stress. The liver responded to oxidative stress through the glycerophospholipid metabolism and choline metabolism in cancer pathways. In conclusion, oxidized fish oil inhibited growth in hybrid grouper and caused oxidative stress and fat accumulation in the liver. This oxidative stress, in turn, changed the hepatic metabolism profile.