文献类型：期刊文献

英文题名：Screening and characterization of sex-linked DNA markers and marker-assisted selection in blue tilapia (Oreochromis aureus)

作者：Wu X.; Zhao L.; Fan Z.; Lu B.; Chen J.; Tan D.; Jiang D.; Tao W.; Wang D.

机构：[1]Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing, 400715, China;[2]Fisheries College, Guangdong Ocean University, Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, China

年份：2021

卷号：530

外文期刊名：Aquaculture

收录：Scopus(收录号：2-s2.0-85091215747)

语种：英文

外文关键词：Blue tilapia; MAS-GMT; Sex-linked markers; WW super-females

外文摘要：The blue tilapia (Oreochromis aureus) is an important cultured fish worldwide, and exhibits sexual size dimorphism in favor of males, and therefore, mono-sex breeding is of great value for aquaculture. However, the lack of accurate sex-linked markers for blue tilapia has hampered the mono-sex culture in aquaculture, as well as study of sex determination in this species. In this study, WW super-females were bred and selected. Then, structure variations between WW and ZZ in sex determining region were identified from blue tilapia by comparative analysis of ZZ/ZW genome re-sequencing data from 5 different strains and ZZ/WW reference genome sequences. Based on these structure variations, 30 pairs of primers were designed to screen sex-linked markers. After two rounds of PCR screening, three markers, named as Marker-1, Marker-2 and Marker-3, were obtained to identify the genetic sex of blue tilapia. Marker-2 and Marker-3 were combined together for multiplex PCR amplification to distinguish ZZ, ZW and WW fish to make up for the lack of a co-dominant marker. In conclusion, three sex-linked markers were isolated and used for identification of different genotypes and establishment of MAS-GMT (marker-assisted selection of genetically male tilapias) technique in blue tilapia. It is also of great significance for isolation of sex determining gene, elucidation of the evolution of sex chromosomes and the transition of sex determination system. ? 2020