文献类型：期刊文献

中文题名：黄芩水提液对肠炎模型小鼠空肠损伤修复的作用

英文题名：Effect of the Scutellaria baicalensis Water Extraction on the Regulation of Jejunum Damage and Repair in the Model of Enteritis Mice

作者：刘晓曦[1];董杰[1,2];李敏霞[1];钱慧慧[1];张继东[1]

机构：[1]广东海洋大学农学院动物医学系,湛江524000;[2]华南农业大学兽医学院,广州510000

年份：2020

卷号：51

期号：2

起止页码：392

中文期刊名：畜牧兽医学报

外文期刊名：ACTA VETERINARIA ET ZOOTECHNICA SINICA

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：国家自然科学基金(31902314);湛江市科技专项(2019B01082);广东海洋大学博士启动费及研究生培养项目(101402/R17088);广东省自然科学基金面上项目(2019A1515011142);广东海洋大学2019年“创新强校工程”(230419057)。

语种：中文

中文关键词：小鼠;肠炎;黄芩;损伤修复

外文关键词：mice;enteritis;Scutellaria baicalensis;damage and repair

中文摘要：本文旨在探讨黄芩水提液对肠炎模型小鼠空肠损伤的作用机理。将30只小鼠随机分为对照组、模型组和黄芩水提液低、中、高剂量组,每组6只小鼠。通过连续3 d给小鼠灌胃0.4 mL·d-1产肠毒大肠杆菌(3×108 CFU·mL-1)建立肠炎模型,黄芩水提液组每只小鼠在攻菌前预灌服0.2 mL黄芩水提液(低剂量组0.3 g·d-1,中剂量组0.6 g·d-1,高剂量组1.2 g·d-1),连续灌胃6 d。结果显示,相比于对照组,模型组小鼠空肠炎性因子IL-6 mRNA表达量极显著升高(P<0.01),空肠绒毛结构受到严重破坏,但参与肠道损伤修复的MAPK mRNA表达量未发生显著变化;相比于模型组,黄芩水提液各剂量组的小鼠空肠IL-6 mRNA表达量极显著下降(P<0.01),空肠JNK/ERK mRNA表达量显著下降(P<0.05),且绒毛结构得到显著改善。综上所述,在小鼠肠炎模型中,黄芩水提液可通过降低炎症反应和下调JNK/ERK mRNA的方式促进空肠的损伤修复。

外文摘要：This paper aimed to discuss the mechanism of the Scutellaria baicalensis water extraction on the damage and repair of jejunum in the model of enteritis mice. Thirty mice were randomly divided into control group, model group, low dose group, medium dose group and high dose group. Each mouse in the model group were fed with 0.4 mL·d-1 enterotoxigenic Escherichia coli(3×108 CFU·mL-1) by the method of intragastric administration for 3 days to establish the model of enteritis. Each mouse in the low(0.3 g·d-1), medium(0.6 g·d-1) and high dose(1.2 g·d-1) groups were pre-administered with the Scutellaria baicalensis water extraction for 6 days before establishing the enteritis model. Results showed that, compared with the control group, the mRNA expression of IL-6 in the jejunum of model group was extremely significantly increased(P<0.01), and the villous structure of jejunum was seriously damaged. However, the mRNA expression of MAPK in the model group that could repair the intestine damage did not change significantly. Compared with the model group, the mRNA expression of IL-6 in the jejunum of Scutellaria baicalensis water extraction groups were all extremely significantly decreased(P<0.01), as well as the mRNA expression of JNK/ERK(P<0.05). Moreover, the villous structures of mice in the Scutellaria baicalensis water extraction groups were all improved. In conclusion, Scutellaria baicalensis water extraction could repair the damage of jejunum by weakening intestinal inflammation and decreasing the mRNA expression of JNK and ERK in the model of enteritis mice.