文献类型：期刊文献

英文题名：Mechanism Analysis of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide from Isochrysis zhanjiangensis Microalgae for Suppressing Vascular Injury in Human Umbilical Vein Endothelial Cells

作者：Chen, Jiali[1];Tan, Li[1];Li, Chengyong[1,2,3];Zhou, Chunxia[1,2,3];Hong, Pengzhi[1,2];Sun, Shengli[1];Qian, Zhong-Ji[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Sch Chem & Environm, Zhanjiang 524088, Guangdong, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524088, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518114, Guangdong, Peoples R China

年份：2020

卷号：68

期号：15

起止页码：4411

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000526304100013)、、WOS

基金：The research was funded by the Natural Science Foundation of Guangdong Province (2020A1515011075) and the Yangfan Scarce Top Talent Project of Guangdong Province (201433009). The research was supported by the 2020 Shenzhen International Scientific and Technological Cooperation R&D Project and funded by the Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1). In addition, the research was funded by the Fund of Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang, ZJW-2019-07).

语种：英文

外文关键词：Isochrysis zhanjiangensis; peptide; angiotensin-I-converting enzyme; HUVEC; in silico

外文摘要：Microalgae are primary producers with multiple nutrients in aquatic environments and mostly have applications in biological feed and fuel industry. There are few studies assessing the angiotensin-I-converting enzyme (ACE) inhibition potential of Isochrysis zhanjiangensis, other than its antioxidant potential. In this study, we evaluated a peptide from I. zhanjiangensis (PIZ, FEIHCC) and its vascular endothelial factors and mechanism in human umbilical vein endothelial cells (HUVEC). The results reveal that PIZ (IC50 = 61.38 mu M) acts against ACE in a non-competitive binding mode. In addition, PIZ inhibits angiotensin II (Ang II)-induced vascular factor secretion and expression by blocking inflammation and apoptosis through nuclear factor kappa B (NF-kappa B), nuclear erythroid 2-related factor 2 (Nrf2), mitogen-activated protein kinases (MAPKs), and the serine/threonine kinase (Akt) signal pathways. This study reveals that PIZ has potential to be developed as a therapeutic agent for hypertension and provides a new method of high-value utilization of I. zhanjiangensis.