文献类型：期刊文献

英文题名：Developmental toxicity, EROD, and CYP1A mRNA expression in zebrafish embryos exposed to dioxin-like PCB126

作者：Liu, Han[1];Nie, Fang-Hong[2];Lin, Hong-Ying[1];Ma, Yi[1];Ju, Xiang-Hong[1];Chen, Jin-Jun[1];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Dept Vet Med, Coll Agr, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Dept Food Safety, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Fac Agr & Life Sci, Lincoln 7647, New Zealand

年份：2016

卷号：31

期号：2

起止页码：201

外文期刊名：ENVIRONMENTAL TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000368034200008)、、EI(收录号：20143600010609)、Scopus(收录号：2-s2.0-84953837349)、WOS

基金：Contract grant sponsor: International Science and Technology Cooperation Project of Guangdong Province, China.; Contract grant numbers: 2007B050200023; 2010B050600004

语种：英文

外文关键词：PCB126; zebrafish embryo; developmental toxicity; EROD; CYP1A

外文摘要：Dioxin-like PCB126 is a persistent organic pollutant that causes a range of syndromes including developmental toxicity. Dioxins have a high affinity for aryl hydrocarbon receptor (AhR) and induce cytochrome P4501A (CYP1A). However, the role of CYP1A activity in developmental toxicity is less clear. To better understand dioxin induced developmental toxicity, we exposed zebrafish (Danio rerio) embryos to PCB126 at concentrations of 0, 16, 32, 64, and 128 g L-1 from 3-h post-fertilization (hpf) to 168 hpf. The embryonic survival rate decreased at 144 and 168 hpf. The fry at 96 hpf displayed gross developmental malformations, including pericardial and yolk sac edema, spinal curvature, abnormal lower jaw growth, and non-inflated swim bladder. The pericardial and yolk sac edema rate significantly increased and the heart rate declined from 96 hpf compared with the controls. PCB126 did not alter the hatching rate. To elucidate the mechanism of PCB126-induced developmental toxicity, we conducted ethoxyresorufin-O-deethylase (EROD) in vivo assay to determine CYP1A enzyme activity, and real-time PCR to study the induction of CYP1A mRNA gene expression in embryo/larval zebrafish at 24, 72, 96, and 132 hpf. In vivo EROD activity was induced by PCB126 at 16 g L-1 concentration as early as 72 hpf but significant increases were observed only in zebrafish exposed to 64 and 128 g L-1 doses (p<0.005) at 72, 96, and 132 hpf. Induction of CYP1A mRNA expression was significantly upregulated in zebrafish exposed to 32 and 64 g L-1 at 24, 72, 96, and 132 hpf. Overall, the severe pericardial and yolk sac edema and reduced heart rate suggest that heart defects are a sensitive endpoint, and the general trend of dose-dependent increase in EROD activity and induction of CYP1A mRNA gene expression provide evidence that the developmental toxicity of PCB126 to zebrafish embryos is mediated by activation of AhR. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 201-210, 2016.