文献类型：期刊文献

英文题名：New mechanisms of biochar-assisted vermicomposting by recognizing different active di-(2-ethylhexyl) phthalate (DEHP) degraders across pedosphere, charosphere and intestinal sphere

作者：Luo, Shuwen[1];Zhen, Zhen[2];Teng, Tingting[3,4];Wu, Weilong[2];Yang, Guiqiong[2];Yang, Changhong[2];Li, Huijun[2];Huang, Fengcheng[2];Wei, Ting[2];Lin, Zhong[1,5];Zhang, Dayi[3,4]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[3]Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China;[4]Jilin Univ, Minist Educ, Key Lab Groundwater Resources & Environm, Changchun 130021, Peoples R China;[5]Guangdong Ocean Univ, Shenzhen Res Inst, Shenzhen 518108, Peoples R China

年份：2023

卷号：458

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：SCI-EXPANDED(收录号：WOS:001037399000001)、、EI(收录号：20232814374787)、Scopus(收录号：2-s2.0-85164257274)、WOS

基金：This work was financially supported by National Natural Science Foundation of China (41977125, 41907033) , Natural Science Founda- tion of Guangdong Province (2022A1515010867, 2022A1515010630, 2019A1515011948) , Shenzhen Fundamental Research Program (JCYJ20220818103609020) . DZ acknowledges support from Chinese Government's Thousand Talents Plan for Young Professionals.

语种：英文

外文关键词：DNA stable isotope probing (DNA-SIP); Biodegradation; Earthworm

外文摘要：Biochar-assisted vermicomposting can significantly accelerate soil DEHP degradation, but little information is known about the underlying mechanisms as different microspheres exist in soil ecosystem. In this study, we identified the active DEHP degraders in biochar-assisted vermicomposting by DNA stable isotope probing (DNASIP) and surprisingly found their different compositions in pedosphere, charosphere and intestinal sphere. Thirteen bacterial lineages (Laceyella, Microvirga, Sphingomonas, Ensifer, Skermanella, Lysobacter, Archangium, Intrasporangiaceae, Pseudarthrobacter, Blastococcus, Streptomyces, Nocardioides and Gemmatimonadetes) were responsible for in situ DEHP degradation in pedosphere, whereas their abundance significantly changed in biochar or earthworm treatments. Instead, some other active DEHP degraders were identified in charosphere (Serratia marcescens and Micromonospora) and intestinal sphere (Clostridiaceae, Oceanobacillus, Acidobacteria, Serratia marcescens and Acinetobacter) with high abundance. In biochar-assisted vermicomposting, the majority of active DEHP degraders were found in charosphere, followed by intestinal sphere and pedosphere. Our findings for the first time unraveled the spatial distribution of active DEHP degraders in different microspheres in soil matrices, explained by DEHP dynamic adsorption on biochar and desorption in earthworm gut. Our work highlighted that charosphere and intestinal sphere exhibited more contribution to the accelerated DEHP biodegradation than pedosphere, providing novel insight into the mechanisms of biochar and earthworm in improving contaminant degradation.