文献类型：期刊文献

中文题名：鲍鱼肠多肽对LPS诱导HUVEC炎症的抑制作用

英文题名：Effect of Abalone Polypeptide on LPS Induced HUVEC Cell Inflammation

作者：萧振邦[1];黄晓洋[1];陈佳丽[2,3];杨胜涛[2];刘怡[1];千忠吉[1,3]

机构：[1]广东海洋大学化学与环境学院,广东湛江524088;[2]广东海洋大学食品科技学院,广东湛江524088;[3]广东海洋大学深圳研究院,广东深圳518108

年份：2020

卷号：40

期号：6

起止页码：108

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：广东省基础与应用基础研究基金项目(2020A1515011075)。

语种：中文

中文关键词：鲍鱼;多肽;HUVEC;抗炎;脂多糖

外文关键词：abalone;peptide;HUVEC;anti-inflammatory;LPS

中文摘要：【目的】探究序列为KVEPQDPSEW的鲍鱼肠多肽AATP对LPS诱导HUVEC人脐静脉内皮细胞的抗炎作用。【方法】用"MTT"法检测细胞活力,"DCFH-DA"法测定活性氧(ROS)的含量,用蛋白免疫印迹法检测细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)和内皮素-1(ET-1)以及NF-κB信号通路相关蛋白的表达情况,酶联免疫实验检测白细胞介素-6(IL-6)的释放量,分子对接分析AATP与Toll样受体(TLR4)蛋白的相互作用。【结果】"MMT"法证明AATP对HUVEC细胞无明显毒性作用(P>0.05),且能提高脂多糖(LPS)诱导组的HUVEC细胞活力(P<0.05);与对照组相比,随着实验组AATP浓度增加,ROS含量明显逐渐减少(P<0.05),内皮炎症因子ICAM-1、VCAM-1、ET-1和IL-6的表达也明显逐渐减少(P<0.05),NF-κB信号通路中p65、I-κBα的磷酸化表达显著下降(P<0.05);对接结果表明,AATP与TLR4能形成稳定的氢键,有相互作用的可能性。【结论】AATP表现出很好的清除ROS作用,可明显抑制HUVEC的炎症因子的表达和NF-κB信号通路的激活。

外文摘要：【Objective】To analyze the anti-inflammatory effect of abalone intestinal polypeptide AATP sequenced KVEPQDPSEW on human umbilical vein endothelial cells(HUVEC)induced by LPS.【Method】MTT method was used to detect cell viability;DCFH-DA was used to detect ROS content;western blotting was used to detect the expression of ICAM-1,VCAM-1,ET-1 and other proteins in the NF-κB signaling pathway,and ELISA test to detect the release of IL-6;molecular docking analysis was used to analyze the interaction between AATP and toll-like receptor(TLR4).【Result】MMT method showed that AATP had no significant toxic effect on HUVEC(P>0.05).It could improve the HUVEC viability of LPS induced group(P<0.05).Compared with the control group,AATP concentration in the experimental group increased,ROS content decreased significantly(P<0.05).The expression of ICAM-1,VCAM-1,ET-1,IL-6 and phosphorylated p65,phosphorylated I-κBαin the NF-κB signaling pathway decreased gradually(P<0.05).The docking results showed that AATP and TLR4 could form stable hydrogen bonds,which had the possibility of interaction.【Conclusion】AATP can clear ROS and inhibit the expression of inflammatory factors and suppress the activation of NF-κB signaling pathway in HUVEC.