文献类型：期刊文献

英文题名：Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis

作者：Guo, Xiaola[1];Wang, Shuai[1];Zhang, Junmei[1];Li, Rui[1];Zhang, Yong'e[1];Wang, Zhengrong[2];Kong, Qingming[3];Cho, William C.[4];Ju, Xianghong[5];Shen, Yujuan[6];Zhang, Lingqiang[7];Fan, Haining[7];Cao, Jianping[6];Zheng, Yadong[8,9]

机构：[1]Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou, Peoples R China;[2]Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China;[3]Hangzhou Med Coll, Sch Biol Engn, Inst Parasit Dis, Hangzhou, Peoples R China;[4]Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Peoples R China;[5]Guangdong Ocean Univ, Coll Agr, Dept Vet Med, Zhanjiang, Peoples R China;[6]Natl Hlth Commiss Peoples Republ China, Key Lab Parasite & Vector Biol, Chinese Ctr Trop Dis Res, Natl Inst Parasit Dis,Chinese Ctr Dis Control & P, Shanghai, Peoples R China;[7]Qinghai Univ, Dept Hepatopancreatobiliary Surg, Qinghai Prov Key Lab Hydatid Dis Res, Affiliated Hosp, Xining, Peoples R China;[8]Zhejiang A&F Univ, Key Lab Appl Technol Green Ecohlth Anim Husb Zhej, Zhejiang Prov Engn Lab Anim Hlth Inspect & Intern, Zhejiang Int Sci & Technol Cooperat Base Vet Med, Hangzhou, Peoples R China;[9]Zhejiang A&F Univ, Coll Vet Med, Hangzhou, Peoples R China

年份：2022

卷号：16

期号：10

外文期刊名：PLOS NEGLECTED TROPICAL DISEASES

收录：SCI-EXPANDED(收录号：WOS:000934966600001)、、Scopus(收录号：2-s2.0-85140414837)、WOS

基金：The study was financially supported by grants from the State Key Laboratory of Sheep Genetic Improvement and Healthy Production (Grant No. MYSKLKF202003 to XG), the Agricultural Science and Technology Innovation Program (Grant No. CAAS-ASTIP-2016-LVRI-03 to SW), and Scientific Research and Development Talent Fund of Zhejiang Agriculture and Forestry University (Grant No. 2021LFR038 to YZ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文摘要：Echinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-derived extracellular vesicles (EVs) of E. multilocularis-infected mice and identified three parasite-origin proteins, thioredoxin peroxidase 1 (TPx-1), transitional endoplasmic reticulum ATPase (TER ATPase), and 14-3-3, being continuously released by the parasites into the sera during the infection via EVs. Using ELISA, both TPx-1 and TER ATPase were shown to have a good performance in diagnosis of experimental murine echinococcosis as early as 10 days post infection and of human echinococcosis compared with that of control. Moreover, TER ATPase and TPx-1 were further demonstrated to be suitable for evaluation of the prognosis of patients with treatment. The present study discovers the potential of TER ATPase and TPx-1 as promising diagnostic candidates for echinococcosis.