文献类型：期刊文献

英文题名：Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge

作者：Wang, Zhongliang[1,2,3,4,5];Wu, Zaohe[1,4,5];Jian, Jichang[1,4,5];Lu, Yishan[1,4,5]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang City 524025, Guangdong, Peoples R China;[2]Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China;[3]Chinese Acad Sci, Grad Sch, Beijing, Peoples R China;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[5]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China

年份：2009

卷号：26

期号：4

起止页码：639

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000266194500010)、、Scopus(收录号：2-s2.0-64549118622)、WOS

语种：英文

外文关键词：SSH; EST; RACE; Bacterial challenge; Real-time PCR

外文摘要：The cDNA of pearl oyster Pinctada fucata Hsp70 (designated PFHsp70) was cloned by EST and rapid amplification of cDNA ends (RACE) techniques. The full length of PFHsp70 cDNA was 2376 bp, consisting of a 5'-terminal untranslated region (UTR) of 89 bp, a 3' terminal UTR of 328 bp, and an open reading frame (ORF) of 1959 bp encoding a polypeptide of 652 amino acids with a theoretical molecular weight of 71.42 kDa and an estimated isoelectric point of 5.18. BLAST analysis revealed that the PFHsp70 gene shared high similarity with other Hsp70 genes. PFHsp70 contained all the three classical Hsp70 family signatures. The results indicated that the PFHsp70 was a member of the heat shock protein 70 family. Fluorescent real-time quantitative RT-PCR was used to examine the expression of PFHsp70 gene in haemocytes of P. fucata after the challenge of bacteria Vibrio alginolyticus. There was a clear time-dependent expression pattern of PFHsp70 after bacterial challenge, and the mRNA expression reached a maximum level at 4 h post-challenge, which returned to control level after 32 h. The up-regulated mRNA expression of PFHsp70 in P. fucata after bacterial challenge indicates that the Hsp70 gene is inducible and involved in immune response. (C) 2008 Published by Elsevier Ltd.