文献类型：期刊文献

英文题名：Effects of Dietary Bile Acids Supplementation on Growth Performance, Sterol Metabolism, Bile Acids Enterohepatic Circulation, and Apoptosis of Juvenile Pacific White Shrimp (Litopenaeus Vannamei)

作者：Li, Xiaoyue[1]; Qu, Kangyuan[1]; Liu, Yucheng[1]; Dong, Xiaohui[1,2,3]; Chi, Shuyan[1,2,3]; Yang, Qihui[1,2,3]; Tan, Beiping[1,2,3]; Zhang, Shuang[1,2,3]; Xie, Shiwei[1,2,3,4]

机构：[1] Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China; [2] Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, 524088, China; [3] Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, 524088, China; [4] Guangdong Provincial Key Lab of Aquatic Animals Disease Control and Healthy Culture, Zhanjiang, 524088, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230158725)

语种：英文

外文关键词：Alcohols - Cell death - Dietary supplements - Fatty acids - Growth rate

外文摘要：Bile acids have an important role in the growth and sterol metabolism of shrimp. The aim of this study was to elucidate the effects of bile acid supplementation in the low fishmeal diets on sterol metabolism, bile acids enterohepatic circulation, bile acid profile, and apoptosis in Penaeus vannamei. Six diets were formulated in this experiment, HFM diet (25% fishmeal), LFM diet (10% fishmeal), and LFM supplemented with four levels (0.15, 0.3, 0.6, 0.9 g kg?1) of bile acids, which were LBA1, LBA2, LBA3, LBA4, respectively. Soy protein concentrate and cottonseed protein concentrate blend was used to replace fishmeal. After 50 days of feeding trial, the shrimp fed the LBA4 diet showed significantly increased final body weight than that fed the LFM diet. The weight gain, survival, and specific growth rate showed no significant differences between the shrimp in LFM and four bile acids supplementary groups. In the sterol metabolism, the shrimp fed the LBA1 diet was significantly increased the HMG-CoA reductase membrane form (hmgcr) and Niemann-Pick type C-2-like protein (npc2) expression compare with that fed the LFM diet, shrimp in LBA2 group was significantly increased the related genes expression [hmgcr and NPC intracellular cholesterol transporter 1-like (npc1)] than those in the LFM group of hepatopancreas. In the lipid metabolism, the shrimp in LBA2 group was significantly increased the carnitine palmitoyltransferase-1 (cpt-1) and AMP-activated protein kinase (ampk) expression than that in the LFM group, shrimp in the LBA3 group was significantly increased the fatty acid binding protein fatty acid (fabp) expression than that in the LFM group. In the bile acids metabolism, the multidrug resistance-associated protein 1-like (mrp2), multidrug resistance-associated protein 2-like (mrp3) and solute carrier family 22 member 7-like (oatp) expressions were significantly increased in LBA1 and LBA2 groups than those in the LFM group of hepatopancreas, organic solute transporter subunit alpha-like (ostα), mrp2, and acyl-coenzyme A thioesterase 1-like (baat) expressions were significantly up-regulated in LBA4 group than those in the LFM group of intestine. Supplementary bile acids in the diet could decrease the apoptosis-related protein expression (BCL-2 and CASPASE3) compare with the shrimp fed low fishmeal diet. The concentration of beta MCA in haemolymph bile acids profile increases with bile acids supplementation. The results showed that bile acids enhanced growth performance, promoted sterol metabolism, enhanced enterohepatic circulation, and reduced apoptosis in shrimp on low fishmeal diets. ? 2023, The Authors. All rights reserved.