文献类型：期刊文献

英文题名：Optimization of peptic hydrolysis parameters for the production of angiotensin I-converting enzyme inhibitory hydrolysate from Acetes chinensis through Plackett-Burman and response surface methodological approaches

作者：Cao, Wenhong[1,2];Zhang, Chaohua[1];Ji, Hongwu[2];Hao, Jiming[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Key Lab Aquat Prod Adv Proc, Guangdong Higher Educ Inst, Zhanjiang 524088, Guangdong, Peoples R China

年份：2012

卷号：92

期号：1

起止页码：42

外文期刊名：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

收录：SCI-EXPANDED(收录号：WOS:000297951200007)、、Scopus(收录号：2-s2.0-82855161407)、WOS

基金：This project was supported by the Science and Technology Exploration Plan Research Funding of Guangdong Ocean University (No. 0912047) and the Earmarked Fund for Modern Agro-industry Technology Research System of China (CARS-47-06B).

语种：英文

外文关键词：Acetes chinensis; enzymatic hydrolysis; angiotensin I-converting enzyme inhibitory activity; Plackett-Burman design; response surface methodology

外文摘要：BACKGROUND: Angiotensin I-converting enzyme (ACE) plays an important physiological role in regulating blood pressure. The elevation of blood pressure could be suppressed by inhibiting ACE. ACE inhibitory peptides derived from food proteins could exert antihypertensive effects without side effects. Acetes chinensis is a marine shrimp suitable for the production of ACE inhibitory peptides. The principal objective of this study was to screen for the significant variables, and further to optimize the levels of the selected variables, for the enzymatic production of ACE inhibitory peptides from Acetes chinensis. RESULTS: Plackett-Burman design and response surface methodology were employed to optimize the peptic hydrolysis parameters of Acetes chinensis to obtain a hydrolysatewith potent ACE inhibitory activity. The peptic hydrolysis variables were subject to a Plackett-Burman design for screening the main factors. The selected significant parameters such as pH, hydrolysis temperature and enzyme/substrate ( E/S) ratio were further optimized using a central composite design. The optimized conditions were: pH 2.5, hydrolysis temperature 45. C, E/S ratio 17 800 U kg-1 shrimp and substrate concentration 200 g L-1. The results showed that 3-5 h hydrolysis could result in a hydrolysate with ACE inhibition IC50 of 1.17 mg mL-1 and a high DH of 25-27%. CONCLUSION: Plackett-Burman design and RSM performed well in the optimization of peptic hydrolysis parameters of Acetes chinensis to produce hydrolysatewith ACE inhibitory activity. A hydrolysatewith potent ACE inhibitory activity and high degree of hydrolysis was obtained, so that the yield of ACE inhibitory peptides in it was high. (C) 2011 Society of Chemical Industry