文献类型：期刊文献

英文题名：Combined Effects of Feed Protein Levels with Cottonseed Protein Concentrate as the Main Protein Source on the Intestinal Flora and Transcriptome of Litopenaeus Vannamei

作者：Wang, Hongming[1]; Hu, Xin[1]; Chen, Jian[1,4]; Zheng, Yudong[1]; Tan, Beiping[1]; Shi, Lili[1]; Zhang, Shuang[1,2,3]

机构：[1] College of Fisheries, Guangdong Ocean University, Zhanjiang, China; [2] Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China; [3] Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, China; [4] Zhanjiang Experimental Station, CATAS, Zhanjiang, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240116452)

语种：英文

外文关键词：Bacteria - Gene expression - Oilseeds

外文摘要：The aim of this study was to reveal the effect of different protein levels (32% (CPC32), 36% (CPC36), 40% (CPC40), 44% (CPC44), and 48% (CPC48)) on the growth and health of Litopenaeus vannamei, using cottonseed protein concentrate (CPC) as the main protein source. The results showed that CPC40 significantly improved the growth performance and lowered the feed coefficient of shrimp compared to CPC32 group; in terms of hepatopancreas biochemistry, CPC40 group significantly increased the antioxidant enzyme activities and gene expression of shrimp. Trypsin and amylase activities were significantly increased in the CPC40 group compared to the CPC32 and CPC48 groups. Meanwhile, different protein levels significantly affected intestinal flora diversity, and the CPC40 group was able to reduce the abundance of harmful bacteria in the gut at the phylum and genus levels. Sequencing and quantitative analyses showed that the expression of HSP90B1 and HSP90AA1 genes was significantly up-regulated in the CPC32 and CPC48 groups compared with the CPC40 group. Correlation analysis showed that the HSP90B1 gene was significantly positively correlated with Photobacterium. In conclusion, the best growth performance of shrimp was achieved at a protein level of 40.01% when CPC was used as the main protein source. high or low protein levels induced the expression of HSP90B1 gene, increased the number of harmful bacteria in the gut, and ultimately reduced the growth performance and health of shrimp. ? 2024, The Authors. All rights reserved.