文献类型：期刊文献

中文题名：莫氏马尾藻(Sargassum mcclurei)褐藻多酚的抗氧化和抗肿瘤细胞增殖作用

英文题名：Antioxidant and Antiproliferative Effects of Phlorotannins from Sargassum mcclurei

作者：卢虹玉[1];刘义[2];邵海艳[1];吉宏武[1]

机构：[1]广东海洋大学水产品深加工广东普通高校重点实验室,广东湛江524088;[2]广东医学院广东天然药物研究与开发实验室,广东湛江524023

年份：2012

卷号：33

期号：23

起止页码：103

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD、、北大核心2011、Scopus、CSCD2011_2012、北大核心、CSCD

基金：广东海洋大学博士启动基金项目(0912111)

语种：中文

中文关键词：莫氏马尾藻;褐藻多酚;抗氧化;抗肿瘤细胞增殖

外文关键词：S.mcclurei; phlorotannins; antioxidant activity; antiproliferative activity

中文摘要：目的:研究莫氏马尾藻(S.mcclurei)褐藻多酚(PTs)的抗氧化活性和抗肿瘤细胞增殖作用。方法:溶剂萃取和超滤的方法进行PTs的提取和精制;Folin-Ciocalteu比色法和磷钼络合物法检测PTs的含量和总抗氧化能力;DPPH自由基和超氧阴离子自由基法检测PTs的自由基清除能力;MTT法检测PTs对sw579甲状腺鳞癌细胞生长抑制作用。结果:获得4种PTs提取物,分别为水层内液(W1)和乙酸乙酯内液(EA1)(分子质量>10kD)、水层外液(W2)和乙酸乙酯外液(EA2)(分子质量<10kD),总酚含量分别为1.672、0.096、1.319、0.143mg没食子酸(GA)/g海藻干基;在相同质量浓度(3mg/L)时,W1的总抗氧化能力最强,相当于2.315mg/L的GA;4种PTs提取物均有清除自由基的作用,W1和EA1的清除作用更强;随着作用时间延长,4种PTs提取物在低质量浓度(4.4mg/L)时对sw579甲状腺鳞癌细胞生长的抑制作用不断增加,其中W2的作用最强,作用72h的抑制率为23.84%。结论:莫氏马尾藻具有显著的抗氧化作用和一定的细胞毒活性,具有潜在的开发应用价值。

外文摘要：Objective： To evaluate the antioxidant and antiproliferation activity of phlorotannins（PTs） from S.mcclurei.Method： S.mcclurei PTs were extracted by solvent extraction and separated by ultrafiltration.PTs content and total antioxidant capacity（TAC） were measured by Folin-Ciocalteu colorimetry and phosphomolybdenum method,respectively.Meanwhile,the scavenging activity against DHHP and superoxide anion radicals were determined and MTT was used to test the growth inhibitory effect of PTs on sw579 thyroid squamous cell carcinoma cells.Results： Four different PTs extracts including aqueous fraction retenate（AR） and filtrate（AF） and ethyl acetate fraction retenate（EAR） and filtrate（EAF） were obtained from the ultrafiltration of two different fractions（aqueous and ethyl acetate） of the crude methanol extract of S.mcclurei through a 10 kD MWCO membrane.The total phenol contents of AR,AF,EAR and EAF were 1.672,0.096,1.319 mg and 0.143 mg of gallic acid per gram of dry matter of S.mcclurei.AR had the strongest TAC at the same concentration（3 mg/L） and was equivalent to 2.315 mg/L of GA.All PTs extracts especially AR and EAR had free radical scavenging effects.Their growth inhibitory effects on sw579 thyroid squamous cell carcinoma cells at a low concentration of 4.4 mg/L increased with increasing treatment time and AF had the most potent antiproliferation activity and showed an inhibitory rate of 23.84% after 72 h treatment.Conclusion： S.mcclurei shows potent antioxidant and antiproliferation activities and thus has great potential for exploitation and utilization.