文献类型：期刊文献

英文题名：Gonadal development and molecular analysis revealed the critical window for sex differentiation, and E₂ reversibility of XY-male spotted scat, Scatophagus argus

作者：Mustapha, Umar Farouk[1];Huang, Yang[1];Huang, Yuan-Qing[1];Assan, Daniel[1];Shi, Hong-Juan[1];Jiang, Mou-Yan[1];Deng, Si-Ping[1];Li, Guang-Li[1];Jiang, Dong-Neng[1]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Famous Fish Reprod Regulat & Breed, Fisheries Coll, Engn Technol Res Ctr, Zhanjiang 524088, Peoples R China

年份：2021

卷号：544

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000685114900003)、、WOS

基金：This study was supported by grants from the Key Project of "Blue Granary Science and Technology Innovation" of the Ministry of Science and Technology (2018YFD0901203); the National Natural Science Foundation of China (Nos. 31972775 and 32002367); Natural Science Foundation of Guangdong Province (2018B030311050); Guangdong Basic and Applied Basic Research Foundation (2019A1515012042 and 2021A1515010430); Department of Education of Guangdong Province (2018KTSCX090).

语种：英文

外文关键词：Gonad development; Sex determination and differentiation; Sex-biased genes; Germ cell; Sex reversal; Spotted scat; (Scatophagus argus)

外文摘要：In vertebrate the development of gonad into testis or ovary requires the combined effect of genes and hormones to trigger differentiation from a bipotential gonadal primordium. Knowledge of the gonad development process is vital in studies on fish sex determination and differentiation. The spotted scat (Scatophagus argus) is an economically important marine fish with an XX/XY sex-determining system and exhibits sexual growth dimorphism in favor of females. Meanwhile, information on gonad development and gene expression patterns in the early gonads is unknown. This study explores the pattern of spotted scat early gonad development by morphological and molecular means. In the undifferentiated gonads, the number of germ cells in females is not significantly different from males. At age 3 mph (months post-hatch), the number of germ cells in females was significantly higher than in males. The sex differentiation began in females before males with the proliferation of primary oocyte and spermatocytes at 3.5 and 4 mph, respectively. In the early gonads, dmrt1 and amh were significantly higher in XY-fish, while cyp19a1a and foxl2 were higher in the XX-fish. Within the critical period of sex differentiation, estradiol treatment was able to sex-reverse XY-male to XY-female. In the sex-reversed group, male (dmrt1 and amh) and female (cyp19a1a and foxl2) related genes were suppressed and increased, respectively. 17 alpha-Methyltestosterone or aromatase inhibitor (letrozole) could not induce sex reversal in XX-fish due to the absence of the male-specific dmrt1 required for testes development. Conclusively, this study identifies the critical window for sex differentiation and significantly higher germ cell numbers in future females than males. We provide evidence that the phenotypic XY-male is reversible, while the phenotypic XX-female is irreversible. Dmrt1 is necessary for testis differentiation, amh in males, and cyp19a1a and foxl2 in females could also be used as molecular markers in early gonads. This study explicitly presents valuable information for research development on sex determination and differentiation and sex control in spotted scat.