文献类型：期刊文献

英文题名：Enhancing shrimp survival and stress response: The role of compound feed attractants in low-temperature conditions

作者：Liao, Zhihong[1,2];Chen, Mengdie[1,2];Gu, Xinyu[1,2];Li, Xiaomin[1,2];Zhang, Wenrui[1,2];Ye, Tao[1,2];Zhao, Wei[1,2];Tan, Beiping[3];Niu, Jin[1,2]

机构：[1]Sun Yat Sen Univ, Sch Life Sci, State key Lab Biocontrol, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Peoples R China;[2]Sun Yat Sen Univ, Sch Life Sci, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Guangzhou, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Nutr & Feed, Zhanjiang 524088, Peoples R China

年份：2026

卷号：611

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001541940100002)、、WOS

基金：This research was supported by the National Key Research and Development Program of China (2023YFD2402000) , and Project of Science and Technology of Guangxi Province (AA23062047) , and Project of Science and Technology of Guangdong Province (2023A1515012627) . We also thank the Home for Researchers ( www.home-for-researchers.com ) .

语种：英文

外文关键词：Low-temperature; Penaeus monodon; Compound feed attractants; Antioxidant capacity; PI3K-AKT

外文摘要：Exposure to low temperatures greatly affects shrimp reproduction, development, and growth, potentially causing high mortality rates and leading to significant economic losses in the global shrimp farming industry. The aim of this study was to investigate the potential protective effects of compound feed attractants (including yeast extracts and enzymatically hydrolyzed plant proteins) against cold stress in Penaeus monodon (P. monodon). Specifically, under low-temperature conditions (19-21 degrees C), three levels of compound feed attractants addition (0, 0.05 %, and 0.1 %) were tested, designated as A0, A0.05, and A0.1, respectively. A total of 360 healthy shrimp (initial weight: 0.49 +/- 0.01 g) were allocated into three groups, with four replicates per group, and fed for eight weeks. The results indicated that compared to the A0 group, the final body weight (FBW), weight gain (WG), specific growth rate (SGR), survival rate (SR), and whole-body crude lipid content were significantly higher in the A0.05 and A0.1 groups, while the feed conversion ratio (FCR) was significantly lower. Higher activities of total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LZM), along with a lower malondialdehyde (MDA) content, were observed in the A0.05 group compared to the A0.1 group. Meanwhile, the activities of digestive enzymes (lipase, protease, and amylase) were significantly higher in the A0.05 and A0.1 groups than in the A0 group. Compared to the A0 group, the A0.05 group optimally preserved hepatopancreatic architecture and improve the integrity of intestinal structure in P. monodon. Gene expression analysis revealed that the A0.05 and A0.1 groups upregulated the expression of genes related to PI3K-AKT (igfr, pi3k, raptor, ppar, akt, 4e-bp), IMD (relish, atg8), AMPs (alf, crustin-2, penaeidin-3), and lipid metabolism (fas, fabp4, ech, elovl4, lcfatp1). Additionally, the A0.05 group had the highest expression levels of gsh-px, Mnsod, cat, and the lowest expression levels of hsp70, hsp90, ldlr. Compared to the A0.05 group, the A0.1 group upregulated the expression of p53 and caspase-3. Overall, 0.05 % compound feed attractants significantly enhances the growth performance, antioxidant capacity, and intestinal health of P. monodon under low-temperature stress, while a 0.1 % dosage may trigger stress responses.