文献类型：期刊文献

英文题名：RNAi knock-down of shrimp Litopenaeus vannamei Toll gene and immune deficiency gene reveals their difference in regulating antimicrobial peptides transcription

作者：Hou, Fujun[1];He, Shulin[1];Liu, Yongjie[1];Zhu, Xiaowen[2];Sun, Chengbo[2];Liu, Xiaolin[1]

机构：[1]Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China

年份：2014

卷号：44

期号：2

起止页码：255

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000341069300001)、、WOS

基金：This work was supported by the Agricultural Science and Technology Achievement Transformation Fund Project of Ministry of Science and Technology, China (No. 2012GB2E200361), the National Fundamental Research Program, China (No. 2006AA10A406), the Key Laboratory of Marine Biology, Institute of Oceanlogy, Chinese Academy of Sciences, Qianjiang distinguished experts of Hangzhou and Extension and Demonstration Program in Desalting Culture of Litopenaeus Vannamei New Breed "Ke Hai 1", Northwest A&F University, China (No. XNY2013-4). Comments from anonymous reviewers have greatly improved the manuscript.

语种：英文

外文关键词：Toll; IMD; Antimicrobial peptides; Litopenaeus vannamei; RNA interference

外文摘要：NF-kappa B dependent antimicrobial peptides (AMPs) are of critical importance in protecting insects or mammals from microorganisms infection. However, we still do not make clear signaling pathways in regulating AMPs expression in shrimps. In this study, RNAi approach was used to study differences between Toll signaling pathway and immune deficiency signaling pathway in regulating the transcription of NF-kappa B dependent AMPs post bacteria challenge. Results showed that the transcription level of anti-lipopolysaccharide factor was highly suppressed in Litopenaeus vannamei immune deficiency (LvIMD) silenced shrimps by gene specific dsRNA compared to Litopenaeus vannamei Toll (LvToll) silenced shrimps with or without Vibrio anguillarum and Micrococcus lysodeikticus challenge. Conversely the transcription level of penaeidin3a was significantly suppressed in LvToll silenced shrimps compared to LvIMD silenced shrimps. However, no obvious difference was found in regulating the transcription of CrustinP. Meanwhile, we found that silencing LvToll both down regulated the transcription of Dorsal and Relish while silencing LvIMD only down regulated the transcription of Relish. At last, shrimp survival experiment showed that post V. anguillarum challenge high mortality was found both in LvToll and LvIMD silenced groups while post M. lysodeikticus challenge we saw high mortality only in LvToll silenced group. Hence, we conclude that shrimp L vannamei Toll pathway and IMD pathway might be different in regulating the transcription of NF-kappa B dependent AMPs and responding to bacteria challenge but not independent of each other. (C) 2014 Elsevier Ltd. All rights reserved.