文献类型：期刊文献

英文题名：Cascading Mechanism Triggering the Activation of Polyphenol Oxidase Zymogen in Shrimp Litopenaeus vannamei After Postmortem and the Correlation with Melanosis Development

作者：Xu, Defeng[1];Yang, Xianqing[2];Wang, Yaling[1];Sun, Lijun[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Key Lab Aq, Guangdong Prov Engn Technol Res Ctr Marine Food, Key Lab Adv Proc Aquat Prod,Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China

年份：2020

卷号：13

期号：7

起止页码：1131

外文期刊名：FOOD AND BIOPROCESS TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000537651500002)、、WOS

基金：The authors received financial support from the National Natural Science Foundation (No. 31201309, 31772048 and 31371777) and the Key Laboratory of Aquatic Products Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute Key Laboratory of Fishery Products Processing, Ministry of Agriculture, People's Republic of China (No. NYJG201404) and the Natural Science Foundation of Guangdong Ocean University (No. C16396).

语种：英文

外文关键词：L. vannamei. Melanization; Hepatopancreas; Cytoarchitecture disruption; SP migration

外文摘要：Melanization is the major deterioration in organoleptic quality of shrimp Litopenaeus vannamei during cold storage and leads to dramatical reduction of commercial value. So far, the biochemical cascade mechanism triggering and accelerating melanosis progression remains unclear. Herein, this investigation aimed to monitor the melanosis development in L. vannamei during 5 days' cold storage at 4 degrees C and to explore the role of serine protease (SP) in zymogen activation of polyphenol oxidase (PPO) by application of low field nuclear magnetic resonance (LF-NMR), transmission electron microscopy (TEM), and immunohistochemistry (IHC). The results showed that melanosis development was positively associated with the increase of PPO and SP activities, and the utmost melanization was observed at cephalothorax segment, following with the telson and abdomen. Besides, LF-NMR relaxometry revealed both a dramatical reduction in trapped water component T22 and a significant increase in free water component T23. Meanwhile, the histopathological findings of hepatopancreas tissue demonstrated the progressive disruption in cytoarchitecture. Along with the increase of T23 and cytoarchitecture disruption, SP and Ca2+ were arbitrarily disseminated in hepatopancreas tissue. In addition, a heat map analysis revealed that there was a highly positive relationship between melanosis development and the aberrant elevation of PPO and SP activities, ratio of T22 components, cytoarchitecture disruption level, and dissemination status of SP and Ca2+. Altogether, the biochemical cascade events for melanosis development of L. vannamei during cold storage could be sketched out; i.e., the cytoarchitecture disruption, in combination with the driving force of free water molecule migration, was greatly favorable for aggregation of SP, Ca2+, and PPO zymogen, following with aberrant activation of PPO zymogen and initiation of melanization. These data provide new insights into the biochemical cascade mechanism of melanosis development in L. vannamei during cold storage and pave new ways for target controlling of melanization at initial stage.