文献类型：期刊文献

英文题名：A high-quality chromosome-level genome assembly of Pacific whiteleg shrimp (Penaeus vannamei)

作者：Liao, Jian[1,2];Lai, Zhuoxin[1,2];He, Chuanmeng[1,2];Li, Gaocong[3];Dong, Zhongdian[1,2];Guo, Yusong[1,2];Wang, Zhongduo[1,2]

机构：[1]Guangdong Ocean Univ, Key Lab Aquaculture South China Sea Aquat Econ Ani, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Coll Elect & Informat Engn, Zhanjiang 524088, Peoples R China

年份：2025

卷号：12

期号：1

外文期刊名：SCIENTIFIC DATA

收录：SCI-EXPANDED(收录号：WOS:001435256600001)、、WOS

基金：This work was funded by Construction Project of Modern Seed Industry Park for Whiteleg Shrimp of Guangdong Province from the Department of Agriculture and Rural Affairs of Guangdong Province, the National Key Research and Development Program of China (Grant No. 2024YFD2401802 & 2024YFD2401803), the Guangdong Provincial Ordinary University Youth Innovative Talent Project in 2024 (Grant No. 2024KQNCX134), the Guangdong Provincial Special Fund Project for Talent Development Strategy in 2024 (Grant No. 2024R3005), the Guangdong Ocean University Scientific Research Startup Funding Project (Grant No. 060302022312), and Guangdong Provincial Field Observation and Research Station for Marine Ecosystem in Hanjiang River Estuary - Nanao Island Area Open Fund (Grant No. HNS202407). Additionally, we would like to express our gratitude to Engineer Li Yijun from Hainan Xinbang Marine Biotechnology Co., Ltd., China, for providing the samples for this project.

语种：英文

外文摘要：The Pacific whiteleg shrimp (Penaeus vannamei, synonym Lipopenaeus vannamei) is an important aquaculture species, and its breeding improvement relies heavily on high-quality genomic resources. Here, we sequenced the genome of the Pacific whiteleg shrimp using PacBio and Hi-C technologies, resulting in a 1.7 Gb genome assembly with a Contig N50 of 57.65Kb and a Scaffold N50 of 605Kb.BUSCO analysis indicated that our genome assembly achieved a gene coverage of 84.6%, a figure considered high among crustacean reference genomes. In total, 907,237,377 bp of repetitive sequences (accounting for 50.48% of the assembled genome) and 22,923 protein-coding genes were identified. Utilizing Hi-C technology, we anchored 14,604 contigs onto 44 chromosomes, yielding a chromosome-level genome assembly with a contig N50 of 42,894,961 bp. In summary, our high-quality genome assembly provides valuable reference genomic resources for the improvement and breeding of elite cultivars of the Pacific whiteleg shrimp.