文献类型：期刊文献

中文题名：四川牦牛UCP1基因遗传多态性及其与肉品质性状的关联分析

英文题名：Genetic polymorphism of UCP1 gene and its correlation with meat quality traits in yak

作者：陆惠娴[1];于海滨[1];谢涛[1];阮梦茹[1];Ambreen Iqal[1];姜平[1];赵志辉[1]

机构：[1]广东海洋大学滨海农业学院,广东湛江524088

年份：2023

卷号：43

期号：2

起止页码：411

中文期刊名：中国兽医学报

外文期刊名：Chinese Journal of Veterinary Science

收录：CSTPCD、、CSCD_E2023_2024、北大核心、CSCD、北大核心2020

基金：国家自然科学基金资助项目(31772562,32002165,32072717);广东省教育厅创新强校工程青年创新人才类资助项目(2019KQNCX042);广东省教育厅创新强校重点平台资助项目(2018302)。

语种：中文

中文关键词：牦牛;UCP1基因;单核苷酸多态性;肉品质性状

外文关键词：yak;UCP1 gene;single nucleotide polymorphism;meat quality

中文摘要：四川牦牛(Bos grunniens,yak)主要分布于我国海拔3000~5000 m的高原地区,以肉质细嫩、味美可口、低脂肪、高蛋白而受到国内外消费者的欢迎。牦牛全身都是宝,藏族人民的衣食住行都离不开它。解偶联蛋白1(uncoupling protein 1,UCP1)是棕色和米色脂肪细胞特异性表达的一种产热线粒体蛋白,通过线粒体中的电子传递链参与底物氧化和ATP分解进程,从而产生热量并为机体供能。本课题组前期通过组学分析发现:UCP1基因可能对脂质代谢具有重要调控作用。因此,本试验拟以四川牦牛为研究对象,探讨UCP1基因在四川牦牛群体中的遗传多态性,分析四川牦牛肉品质性状相关的潜在遗传标记位点。结果显示,UCP1基因的内含子4和外显子5中共发现4个SNPs位点,其中I4-769 G>A位点的AA型的背最长肌红度显著高于GG型;在I4-836 C>T位点中CC型的背最长肌红度和黄度显著高于CT型;E5-26 C>G位点的CC型的背最长肌亮度显著低于CG型,而CC型背最长肌红度和黄度显著高于CG型;E5-59 A>G位点的GA型的背最长肌24 h时的pH值显著高于AA型。单倍型分析结果表明:共有6种单倍型,其中H1H1和H1H2单倍型组合的背最长肌亮度显著低于H1H3单倍型组合;H2H2单倍型组合的背最长肌红度和黄度显著高于H1H3单倍型组合;而H2H2单倍型组合的背最长肌黄度显著高于H1H3和H2H3单倍型组合。本研究为选育优质肉品质性状牦牛提供有利的分子遗传标记,为进一步阐明UCP1基因的功能奠定基础。

外文摘要：The yak(Bos grunniens)is mainly distributed in the plateau areas of China at an altitude of 3000-5000 m.It has the advantages of rough feeding,strong cold resistance,and strong adaptability to low-oxygen environments.It mainly feeds on organic green pastures and is green and healthy food for the Tibetan people.Uncoupling protein 1(uncoupling protein 1,UCP1)is a member of the uncoupling protein family.It is a heat-producing mitochondrial protein specifically expressed by brown and white adipocytes,which participates in substrate oxidation and ATP breakdown through the electron transport chain in mitochondria,thereby generating heat and supplying energy to the body.This experiment took Sichuan yak as the research object to explore the genetic polymorphism of the UCP1 gene in the Sichuan yak population,and analyze the potential genetic markers related to meat quality traits of Sichuan yak.The results showed that:a total of 4 SNPs were found in intron 4 and exon 5 of the UCP1 gene,and they were all in Hardy Weinberg equilibrium.The redness(a~~*)of the longissimus dorsi of the type AA at the I4-769 G>A site was significantly higher than that of the type GG;the redness(a*)of the longissimus dorsi of the type CC at the I4-836C>T site and yellowness(b*)was significantly higher than that of type CT;the brightness(L*)of the type CC at the E5-26C>G site was significantly lower than that of the type CG,while the redness(a*)and yellowness(b*)of the longest dorsal muscle were significantly higher than those of the type CG;the longissimus dorsi of the type GA at the E5-59A>G site had a significantly higher pH at 24hthan the type AA.Analysis of the haplotype results showed that the longissimus dorsi brightness(L*)of the H1H1and H1H2haplotype combination was significantly lower than that of the H1H3haplotype combination;the longissimus dorsi redness(a*)and the H2H2haplotype combination.The yellowness(b*)was significantly higher than the H1H3haplotype combination;the longissimus dorsi yellowness(b*)of the H2H2haplotype combination was significantly higher than the H1H3and H2H3haplotype combination.In summary,the H2H2haplotype can be used as the best haplotype to improve the quality of Sichuan yak meat.This study can provide promising molecular genetic markers for breeding high meatquality yak and provide an important theoretical basis for the molecular marker-assisted breeding of Sichuan yak.