文献类型：期刊文献

英文题名：Genome-wide characterization of the Hsp40 gene family in Clarias fuscus reveals their roles in thermal stress adaptation and immune modulation

作者：Ye, Minghui[1,2];Zhou, Dayan[3];Kong, Lingwei[1,2];Zhang, Yulei[1,2];Pan, Chuanhao[1,2];Huang, Cailin[3];Li, Guangli[1,2];Chen, Huapu[1,2];Tian, Changxu[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Res Ctr Reprod Control & Breeding Techno, Guangdong Prov Engn Lab Mariculture Organism Breed, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[3]Guangxi Intro & Breeding Ctr Aquaculture, Nanning 530001, Peoples R China

年份：2026

卷号：57

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

收录：SCI-EXPANDED(收录号：WOS:001607387100001)、、WOS

基金：This work was supported by the Guangdong Basic and Applied Basic Research Foundation (2025A1515012346) , the Key Research and Development Task Special Project of the Xinjiang Uygur Autonomous Region (2024B02014) , the Department of Education of Guangdong Province (2023KTSCX042) , the Zhanjiang Science and Technology Plan Project (2025R02111) , the Postgraduate Education Innovation Project of Guangdong Ocean University (202535) , the Modern Seed Industry Park for Whiteleg Shrimp of Guangdong Province (K22221) , the Undergraduate Innovation Team Project of Guangdong Ocean University (CXTD2023003) , the Youth Science and Technology Innovation Talent of Guangdong TeZhi plan talent (2023TQ07A888) , and the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (2024CXTD26) .

语种：英文

外文关键词：Clarias fuscus; Heat shock protein 40; Gene family identification; High temperature stress

外文摘要：Thermal stress poses a significant challenge to fish physiology and aquaculture sustainability, necessitating an indepth understanding of molecular responses to temperature fluctuations. This study characterizes the Hsp40 gene family in Hong Kong catfish (Clarias fuscus), a species known for its thermal resilience, to elucidate their roles in proteostasis and immune modulation. A total of 55 Hsp40 genes were identified and categorized into three subfamilies: DnaJa (n = 6), DnaJb (n = 16), and DnaJc (n = 33). These genes exhibit conserved J-domains and diverse motifs, indicating adaptations to aquatic stressors. Phylogenetic analysis reveals that the Hsp40 gene family in C. fuscus exhibits the closest evolutionary relationship to Ictalurus punctatus, followed by Danio rerio, with distinct clustering patterns across the subtypes. Collinearity and synteny analyses indicated substantial genomic conservation of Hsp40 genes between C. fuscus, I. punctatus, and D. rerio, with evidence of gene duplication and homologous relationships. The subcellular localization of Hsp40 proteins was observed in the cytoplasm, endoplasmic reticulum, mitochondria, nucleus, and extracellular regions, supporting their roles in chaperone activity and immune response regulation. Protein interaction networks identified two major clusters: one associated with protein folding and another with Hsp90-dependent immune signaling. Molecular docking confirmed stable interactions between Hsp40 and Hsp70, aiding in the mitigation of thermal-induced protein misfolding. Transcriptomic analysis under thermal stress showed significant differential expression of 30 Hsp40 genes, with dnajb11 and dnajc5.1 notably differentially expressed in the liver, gills, and kidneys. Under normal-temperature conditions, 10, 6, and 12 genes were differentially expressed in the liver, gills, and kidneys, respectively. High-temperature acclimation significantly increased the number of differentially expressed genes in the kidneys (20 genes), indicating enhanced thermal tolerance. These genes bolster immunity by stabilizing proteins and modulating inflammatory pathways. These findings suggest that key Hsp40s, such as dnajb11 and dnajc5.1, serve as biomarkers for selective breeding and support the development of strategies like immunomodulatory feed additives to enhance thermal resilience in C. fuscus aquaculture.